| 姚苹苹,陈钢,徐芳,杨章女,陈晨,孙一晟,卢杭景,庞卫龙,张云,朱函坪,项海青.浙江省天台县2011－2018年汉坦病毒基因型别和进化变异研究[J].Chinese journal of Epidemiology,2019,40(10):1285-1290 |
| 浙江省天台县2011－2018年汉坦病毒基因型别和进化变异研究 |
| Genotype and evolution of hantavirus in Tiantai of Zhejiang province, 2011-2018 |
| Received:February 05, 2019 |
| DOI：10.3760/cma.j.issn.0254-6450.2019.10.021 |
| KeyWord: 汉坦病毒 基因型 进化变异 流行特征 |
| English Key Word: Hantavirus Genotype Gene variation Epidemiological characteristic |
| FundProject:国家自然科学基金（81473036，81171609）；国家重点研发计划（2017YFC1601503）；国家科技重大专项（2018ZX10714002） |
| Author Name | Affiliation | E-mail | | Yao Pingping | Institute of Microbiological Test, Key Laboratory of Vaccine, Prevention and Control on Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | | | Chen Gang | Institute of Microbiological Test, Key Laboratory of Vaccine, Prevention and Control on Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | | | Xu Fang | Institute of Microbiological Test, Key Laboratory of Vaccine, Prevention and Control on Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | | | Yang Zhangnyu | Institute of Microbiological Test, Key Laboratory of Vaccine, Prevention and Control on Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | | | Chen Chen | Institute of Microbiological Test, Key Laboratory of Vaccine, Prevention and Control on Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | | | Sun Yisheng | Institute of Microbiological Test, Key Laboratory of Vaccine, Prevention and Control on Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | | | Lu Hangjing | Institute of Microbiological Test, Key Laboratory of Vaccine, Prevention and Control on Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | | | Pang Weilong | Tiantai Municipal Center for Disease Control and Prevention, Tiantai 317200, China | | | Zhang Yun | Institute of Military Medicine, Nanjing Command, Nanjing 210002, China | | | Zhu Hanping | Institute of Microbiological Test, Key Laboratory of Vaccine, Prevention and Control on Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China | hpzhu@cdc.zj.cn | | Xiang Haiqing | Hangzhou Municipal Center for Health Development, Hangzhou 310001, China | xhq87065651@163.com |
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| Abstract: |
| 目的 研究2011－2018年肾综合征出血热（HFRS）国家监测点浙江省天台县汉坦病毒（HV）的基因型别和进化变异情况，了解天台县HV分子流行病学特点。方法 将天台县2011－2018年HV抗原阳性的鼠肺标本超声后提取核酸，利用HV型特异性引物，应用巢式PCR对部分M片段进行扩增分型和序列测定，将天台县2011－2018年的HV序列与国内外其他已知的HV序列进行比较，以明确该地区的基因型别，分析病毒的进化变异情况。结果 HV抗原阳性的67份鼠肺标本经型特异性引物巢式PCR扩增后31份标本为阳性，其中30份为汉滩病毒（HTNV）、1份为汉城病毒（SEOV），31份PCR阳性鼠肺均来自黑线姬鼠。31份巢式PCR阳性产物的部分M片段核苷酸序列有30株分布在HTNV单元群，1株分布在SEOV单元群。HTNV单元群中的天台T2018-130株与天台其余29株及国内外其他株同源性为84.8%～87.9%，差异较大，天台其余29株亲缘关系较近； SEOV发生群中的T2016-31株来自黑线姬鼠的鼠肺标本，与SEOV天台以往分离株ZT71株、ZT10株和浙江温州分离株Z37株在系统发生树上分布于同一或临近分支。结论 浙江省天台县HV流行的主要型别HTNV基因表现出明显的地理聚集现象，但也存在着基因差异较大的变异株T2018-130株；同时从病毒基因序列分析证实SEOV T2016-31株存在于黑线姬鼠中，可能意味着在SEOV进化过程中病毒在宿主间发生了“溢出”现象。 |
| English Abstract: |
| Objective By investigating the genotype and evolutionary variation of hantavirus (HV) in Tiantai county, a national surveillance site for hemorrhagic fever with renal syndrome (HFRS) was set in Zhejiang province, from 2011 to 2018, to reveal the molecular epidemiological characteristics of hantavirus (HV) in Tiantai. Methods Total RNA was extracted from ultrasound treated HV antigen- positive rat lung samples in Tiantai from 2011 to 2018. After cDNA was prepared, nested PCR was used to amplify partial sequence of M fragments by using specific primers of HV. The sequences of HV in Tiantai from 2011 to 2018 were compared with other known HV sequences in order to identify the genotype and analyze the evolution and variation of the virus. Results In 67 HV antigen-positive lung specimens, 31 were positive in nested PCR amplification with type-specific primers, including 30 Hantaan virus (HTNV) positive samples, 1 Seoul virus (SEOV) positive sample, and all the 31 samples were from Apodemus agrarius. The phylogenetic tree based on partial M segment was divided into monophyletic group, 30 strains were distributed in HTNV group and 1 was in SEOV group. The HTNV strain Tiantai T2018-130 was independently in one branch, sharing 84.8%-87.9% homology with other strains both at home and abroad, including 29 strains in HTNV group in Tiantai. The other 29 HTNV strains in Tiantai showed closer relationship. The SEOV strain T2016-31 from Apodemus agrarius showed closer relationship with previous strains of SEOV, Tiantai ZT71, ZT10 and Z37 strains of Wenzhou, Zhejiang province. Conclusions HTNV, the main genotype of HV in Tiantai of Zhejiang province, showed obvious geographic clustering, but the strain T2018-130 was distinct from the others in Tiantai. Meanwhile, by sequence analysis, we confirmed that The SEOV strain T2016-31 existed in in Apodemus agrarius, indicating there was a phenomenon of "spillover" between virus and host in SEOV evolution. |
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