无锡市2013－2016年HIV-1亚型及进化分析

殷玥琪; 陈剑双; 成浩; 张轩; 吴楠楠; 陈璐斯; 王蓓

Abstract

殷玥琪,陈剑双,成浩,张轩,吴楠楠,陈璐斯,王蓓.无锡市2013－2016年HIV-1亚型及进化分析[J].Chinese journal of Epidemiology,2020,41(2):244-248

无锡市2013－2016年HIV-1亚型及进化分析

Transition and evolution of HIV-1 subtype among HIV-1 infections in Wuxi city, 2013-2016

Received:May 13, 2019

DOI：10.3760/cma.j.issn.0254-6450.2020.02.019

KeyWord: HIV-1型亚型进化

English Key Word: HIV-1 Subtype Evolution

FundProject:中央高校基本科研业务费专项资金（2242016K40025）；江苏省研究生科研与实践创新计划（KYCX17_0184）

Author Name	Affiliation	E-mail
Yin Yueqi	Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China
Chen Jianshuang	Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China
Cheng Hao	Wuxi Xinwu District Center for Disease Control and Prevention, Wuxi 214028, China
Zhang Xuan	Wuxi Center for Disease Control and Prevention, Wuxi 214000, China
Wu Nannan	Department of Healthcare Associated Infection Control, Wuxi Second People's Hospital, Wuxi 214002, China
Chen Lusi	Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China
Wang Bei	Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China	wangbeilxb@163.com

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Abstract:

目的了解无锡市HIV-1亚型流行及进化特征，为预测本地HIV-1疫情变化提供参考依据。方法样本来源于2013年4月至2016年7月无锡市部分CD₄⁺T淋巴细胞监测队列，进行HIV-1基因的扩增和测序，采用ChromasPro 1.6和MEGA 7.0软件构建HIV-1序列数据库；采用FastTree 2.1.10和BEAST 1.7.2软件和贝叶斯系统进化推断法重构HIV-1历史传播情况，采用SPSS 22.0软件进行统计学分析。结果有205例HIV-1感染者，其中≥50岁占32.68%（67/205）。共检测出CRF01_AE、CRF07_BC、CRF67_01B、B、CRF08_BC、CRF68_0B、CRF78_ cpx 7种HIV-1基因型及1例独特重组型。流行亚型以CRF01_AE（51.67%，93/180）及CRF07_BC（17.22%，31/180）为主，不同亚型之间传播方式的差异有统计学意义（χ²=16.99，P≤0.05）。CRF67_01B型（12.78%，23/180）所占比例较高。贝叶斯系统进化推断法分析结果显示，无锡市CRF67_01B型进化率为2.29×10^-3，最近共同祖先时间约为2 003.10年，与江苏省及安徽省来源的参考株可能存在亲缘关系，CRF67_01B型于2003年开始在无锡市出现传播。结论 2013－2016年无锡市HIV-1亚型复杂多样，CRF67_01B型已经开始在无锡市流行，应持续监测HIV-1亚型变化，从分子角度为疫情预测提供参考依据。

English Abstract:

Objective To understand the distribution of HIV-1 subtype in Wuxi city, to predict the local HIV-1 epidemics. Methods Samples were collected from the ‘CD₄⁺T lymphocyte monitoring cohort study’ in Wuxi from April 2013 to July 2016. HIV-1 gene was amplified, sequenced and with HIV-1 sequence database constructed, using both the ChromasPro 1.6 and MEGA 7.0 softwares. Bayesian phylogenetic inference was used to rebuild the history of HIV-1 transmission, while BEAST 1.7.2 and FastTree 2.1.10 software were used for data analysis. Statistical analysis using SPSS 22.0 software. Results Among the 205 subjects of HIV-1 infection, 32.68%(67/205) of them were over 50 years old. Seven subtypes (including CRF01_AE, CRF07_BC, CRF67_01B, B, CRF08_BC, CRF68_0B, CRF78_cpx) and one of the unique recombinant forms (URFs) were detected. The main subtypes were CRF01_AE (51.67%, 93/180) and CRF07_BC (17.22%, 31/180). Differences between subtypes and ways of transmission were statistically significant (χ²=16.99,P≤0.05). The proportion of CRF67_01B (12.78%, 23/180) was higher than before. Results from Bayesian phylogenetic inference analysis showed that the evolution rate was 2.29×10^-3 and Time to the Most Recent Common Ancestor (tMRCA) was 2 003.10. CRF67_01B was probably related to the reference strains from Jiangsu and Anhui provinces, and had been spreading in Wuxi since 2003. Conclusions HIV-1 subtypes seemed complex and diverse in Wuxi city in 2013-2016, with CRF67_01B being pervasive. Continuous molecular monitor program was still needed to provide reference for the prediction of epidemics, from the molecular perspective.

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