| 蒋力云,曹毅敏,许杨,景钦隆,曹庆,狄飚,杨智聪.广州市2011年登革病毒流行状况及E基因进化特征分析[J].中华流行病学杂志,2012,33(12):1273-1275 |
| 广州市2011年登革病毒流行状况及E基因进化特征分析 |
| Epidemiological situation and the E gene evolution of dengue virus in Guangzhou, 2011 |
| 收稿日期:2012-05-28 出版日期:2014-09-18 |
| DOI：10.3760/cma.j.issn.0254-6450.2012.12.017 |
| 中文关键词: 登革病毒 E基因 流行特征 |
| 英文关键词: Dengue virus E gene Epidemiological characters |
| 基金项目:国家自然科学基金(81271880，81273139)；广州市卫生局重点项目(201 102A212006) |
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| 中文摘要: |
| 目的 了解2011年广州市登革热的流行情况,分析新分离毒株的E基因分子特征.方法 收集2011年广州市登革热的流行病学资料和血清标本.采用荧光定量PCR检测并确定血清型,C6/36细胞进行病毒分离,测定新分离毒株的E基因序列,利用Mega 4.0软件分析.结果 2011年广州市登革热发病高峰在9-11月.在患者血清中检测出登革热病毒1、2、4型,分离出5株登革热1型毒株.在基因型上,4株属于亚洲型,1株属于美洲/非洲型.结论 广州市登革病毒与东南亚地区的毒株有较高同源性,且存在登革热暴发的潜在风险,该病毒在广州市可能已出现本地化趋势. |
| 英文摘要: |
| Objective To investigate the epidemiological characteristics of Dengue and the E gene of the new isolated strains.Methods Epidemiological data and serum samples were collected.Serotypes were detected by real-time PCR and virus was isolated in C6/36.E gene of the new isolated strains were sequenced and analyzed by Mega 4.0.Results The cases of Dengue reached at the peak during September and November,with Serotype 1,2 and 4 were involved.Five strains of serotype 1 were isolated,with 4 of them fell into the clad of Asia genotype,and 1 belonged to America/Africa genotype.Conclusion The strains isolated in Guangzhou showed a high identity to the Southeast Asian strains.There seemed high risk of outbreak of Dengue in this area,However,the Dengue virus might have already been localized. |
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