| 刘贞艳,房明,胡彬,毕振旺,寇增强,任艳艳,陈保立,毕振强.山东省2013年致猩红热及无症状携带者A群链球菌分子分型研究[J].中华流行病学杂志,2014,35(12):1375-1378 |
| 山东省2013年致猩红热及无症状携带者A群链球菌分子分型研究 |
| Molecular types of group A Streptococcus isolated from scarlet fever patients and asymptomatic carriers in Shandong province,2013 |
| 收稿日期:2014-07-28 出版日期:2014-12-10 |
| DOI：10.3760/cma.j.issn.0254-6450.2014.12.014 |
| 中文关键词: 猩红热 链球菌,酿脓 分子分型 |
| 英文关键词: Scarlet fever Streptococcus pyogenes Molecular types |
| 基金项目:国家科技重大专项(2012ZX10004215) |
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| 中文摘要: |
| 目的 描述2013年山东省致猩红热及无症状携带者A群链球菌(GAS)分子分型特征,探讨各分型方法与emm型别的关联.方法 自猩红热、无症状携带者咽拭分离获得GAS 72株,利用emm、多位点序列分型(MLST)、超抗原(SAg)基因(speA、speC、speG、speH、speI、speJ、smeZ、ssa、speK、speM、speL)检测和脉冲场凝胶电泳(PFGE)对其进行分型检测.结果 72株GAS中,以emm1型(41.67%)和emm12型(56.94%)为主;共检出两种ST型别,其中ST28型31株(43.06%),ST36型41株(56.94%);ST28型与emm1相关(P<0.05),而ST36型与emm12相关(P<0.05);speA、speC、speG、speH、speI、speJ、smeZ、ssa的检出率分别为38.89%、95.83%、97.22%、38.89%、54.17%、41.67%、100.00%、100.00%,speK、speM、speL未检出;emm1型菌株倾向于含有speA、speJ,不含有speH、speI(P<0.05);emm12恰好相反,倾向于含有speH、speI,不含有speA、speJ(P<0.05);PFGE共检出20种型别.结论 2013年山东省致猩红热和无症状携带者中GAS型别分布较为单一,emm型别以emm1和emm12为主;ST型别主要为ST28和ST36,超抗原基因携带以speC、speG、smeZ、ssa为主.各分型方法间存在一定关联. |
| 英文摘要: |
| Objective To describe the molecular characteristics of group A Streptococcus (GAS) isolated from patients and asymptomatic carriers of scarlet fever in Shandong province,2013,and to explore the relationships between emm types and other molecular types. Methods 72 strains of GAS were isolated from throat swabs of children with scarlet fever or asymptomatic carriers of GAS. All the strains were typed by emm typing,multilocus sequence typing (MLST),super-antigen (SAg) genes detections and pulsed-field gel electrophoreses (PFGE). Results Among the 72 strains,emm1(41.67%) and emm12 (56.94%) were the most common emm types. Two ST types were found,including ST28 (43.06%) and ST36 (56.94%). Additionally,emm1 was also found correlated to ST28,while emm12 was associated with ST36. Eight super-antigen genes were detected,including smeZ(100.00%),ssa(100.00%),speG(97.22%),speC(95.83%),speL(54.17%),speJ(41.67%),speA (38.89%) and speH (38.89%),while speK,speM,speL were not found (0%). Both speA and speJ genes were detected primarily in emm1 strains (all P<0.05),while speH and speI genes were not detected in emm 1 strains (all P<0.05). And emm12 strains were inclined to harbor speH and speL (all P<0.05) but not speA or speJ (all P<0.05). Twenty different genotypes were identified by PFGE. Conclusion All the emm types of GAS isolated from scarlet fever patients and asymptomatic carriers in Shandong province 2013 were mainly emm1 and emm12 and carrying speC,speG and smeZ,ssa. ST types mainly exsited in ST28 and ST36. In addition,there were correlations between emm types and super-antigen genes,ST types,PFGE types. |
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