文章摘要
何军,刘丽萍,侯赛,龚磊,吴家兵,胡万富,王建军.安徽省2株人感染H9N2流感病毒基因特征[J].中华流行病学杂志,2016,37(5):708-713
安徽省2株人感染H9N2流感病毒基因特征
Genomic characteristics of 2 strains of influenza A (H9N2) virus isolated from human infection cases in Anhui province
收稿日期:2015-12-15  出版日期:2016-05-13
DOI:10.3760/cma.j.issn.0254-6450.2016.05.025
中文关键词: H9N2流感病毒  全基因组  序列比对  氨基酸位点
英文关键词: H9N2 influenza virus  Genome  Sequence alignment  Amino acid position
基金项目:安徽省科技合作计划项目(1503062008);上海市科委计划项目(14495810301)
作者单位E-mail
何军 230601 合肥, 安徽医科大学公共卫生学院
230601 合肥, 安徽省疾病预防控制中心微生物检验科 
 
刘丽萍 230601 合肥, 安徽省疾病预防控制中心微生物检验科  
侯赛 230601 合肥, 安徽省疾病预防控制中心急性传染病防治科  
龚磊 230601 合肥, 安徽省疾病预防控制中心急性传染病防治科  
吴家兵 230601 合肥, 安徽省疾病预防控制中心急性传染病防治科  
胡万富 230601 合肥, 安徽省疾病预防控制中心微生物检验科  
王建军 230601 合肥, 安徽医科大学公共卫生学院 wjj@ahcdc.com.cn 
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中文摘要:
      目的 分析2015年安徽省2株人感染H9N2禽流感病毒分离株全基因组特征。方法 对安徽省2015年4月和9月流感监测网络实验室先后发现并确诊的2例H9N2禽流感病毒感染病例及其病毒分离株全基因组序列进行分析,使用Mega 6.0等软件解析2株H9N2毒株全基因组特征。结果 安徽省首次报道人感染H9N2禽流感病毒病例,对病毒分离株分析显示,其HA和NA基因与中国2013年禽间流行的H9N2病毒高度同源,属于A/Chicken/Shanghai/F/98(H9N2)支系;而PB2和MP基因属于A/quail/Hong Kong/G1/97支系,与H7N9、H10N8和H6N2具有较高的相似度。氨基酸序列比对发现该2株病毒出现多个人易感倾向位点分子特征:HA蛋白发生Q226L、H183N和E190T突变,且HA裂解位点为PSRSSR\GL排列,NA蛋白茎部发生63~65位氨基酸缺失,M2蛋白S31N突变,以及PA-100A、PA-356R和PA-409N。结论 安徽省首次报道的人感染禽源H9N2流感病毒为H9N2系间重配病毒,存在多个人易感位点。
英文摘要:
      Objective To understand genomic characteristics of 2 strains of influenza A (H9N2) virus isolated from human infection cases in Anhui province in 2015. Methods Two human infection with H9N2 virus were confirmed by national influenza surveillance laboratory network in Anhui through viral isolation in April and September, 2015, respectively. The full genomic sequences of the two viral isolates were analyzed in this study by using molecular bioinformatics software Mega 6.0. Results Human infection with H9N2 virus was first reported in Anhui province. The analysis of genomic sequence showed that the HA and NA genes of the two H9N2 isolates belonged to A/Chicken/Shanghai/F/98(H9N2)-like lineage, and shared high identity with H9N2 virus circulating in poultry in 2013. The PB2 and MP genes belonged to the A/quail/Hong Kong/G1/97-like lineage, and shared high homology with H7N9, H10N8 or H6N2 viruses. The amino acid sequence alignment results showed that several mutations for human infection tropism presented in the two virus strains, including Q226L, H183N and E190T in HA; S31N in M2; 63-65 deletion in NA. In addition, the H9N2 influenza virus strains possessed the PSRSSR\GL motif in HA. Meanwhile several human-like signatures, including PA-100A, PA-356R and PA-409N were also found in the two virus strains. Conclusion The H9N2 viruses isolated from human infection cases in Anhui province belonged to a reassortant virus originated from different lineage H9N2 avian influenza virus. The virus has possessed several human susceptibility locus.
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