| 张挺秀,代翔,米吉提.酶联免疫吸附试验检测鼠疫抗体实验研究[J].Chinese journal of Epidemiology,1983,4(2):116-119 |
| 酶联免疫吸附试验检测鼠疫抗体实验研究 |
| ELISA as a Tool for Detection of Anti-plague Antibody |
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| Abstract: |
| 1980年作者等应用ELISA间接法对189份长尾黄鼠血清和217份灰旱獭血清检测了鼠疫抗体,并与间接血凝试验(PHA)比较。结果ELISA法检出黄鼠阳性血清44份,旱獭8份。而PHA法检出黄鼠阳性血清25份,旱獭2份。经多次重复试验,结果相同,证明ELISA滴度高于PHA。用鼠疫强毒菌和EV菌分别以7~10亿菌/毫升给黄鼠皮下接种,经14~35天后,可产生血凝素,个别的黄鼠可产生较高的血凝素和ELISA高滴度。从而为检测鼠疫抗体提供了一种新的敏感有效的技术方法。 |
| English Abstract: |
| In 1980,189 sera samples from citellus undulatus pallas and 217 from marmota baibacina Brandt were tested for anti-plague antibody with ELISA. PHA was used parailelly as control.ELISA detected out 44 positive samples from sera of Citellus and 8 positive samples from marmota, whereas by PHA method only 25 from Citellus and 2 from marmota. By repeating tests, the same results were obtained, which indicated higher sensitivity with ELISA. After subcutaneous inoculation of Citellus with 7×108-1×109 cells of virulent or EV strain of Yersinia Pestis, 14—35 days later hemagglutinin was revealed. In certain individuals of Citellus undulatus Pallas high titre of hemagglutinin and ELISA-antibody were found.The author believed that ELISA provides a new tool in plague surveillance as being more sensitive and effective in detecting specific antibodies. |
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