| 蔡保健,李烽,刘学礼,严玉辰,孟庆海,李志仁,孔令雄.流行性出血热病毒的形态研究:Ⅰ 在人二倍体细胞内病毒繁殖的电镜观察[J].Chinese journal of Epidemiology,1983,4(3):137-139 |
| 流行性出血热病毒的形态研究:Ⅰ 在人二倍体细胞内病毒繁殖的电镜观察 |
| Studies on the Morphology of Epidemic Haemorrhagic Fever Ⅰ |
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| Abstract: |
| 本文报道了EHF病毒适应人胚肺二倍体细胞的电镜形态。EHF病毒在细胞质内,有细胞膜及空泡芽生形式。在感染细胞内外都看到病毒颗粒,为球形及椭圆形有包膜的病毒,有致密和疏松两种颗粒,直径分别为110~150毫微米和120~160毫微米,偶见80~100毫微米的病毒颗粒,最大的可达200毫微米左右。本病毒比布尼亚病毒大的多。因此与典型布尼亚病毒既有类似也有差异。 |
| English Abstract: |
| EHF virus was detected by electron microscopy in ultrathin section of diploid cell lines of human embryonic lung (2BS).It appeared in spherical or elliptic form with envelope both intra and extracellularly.Particles localizing in cytoplasma and budding from cytoplasmic membrane and vesicles were observed.Two types of virions were found.The smaller type particles (110-150 nm in diameter) had a markedly electron-dense contents and the bigger type particles (120-160nm) were of moderate electron density. The maximum particles reached about 200nm and the minute one ranged from 80-100nm occasionally. EHF virus was much bigger than common Bunyavirus.It was similar to classical Bunyavirus in some respects, but quite different from Bunyavirus in other respects. |
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