| 李忠元,张旭帆,张秀琴,胡淑敏,刘秉阳.一种简便的厌氧菌培养法[J].Chinese journal of Epidemiology,1983,4(3):181-184 |
| 一种简便的厌氧菌培养法 |
| A Simple Method of Culturing Anaerobe |
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| Abstract: |
介绍用简便的缸内法培养厌氧菌,方法是用干燥试剂的耐压玻缸,其底部置铁屑或钢丝绒,通过化学反应吸收游离氧,在输入适量CO2后密封置温箱内培养肠道厌氧菌,此法无需特殊设置,一般试验室即可应用。我们自制的无氧指示剂与国外的B.B.L.Gaspak厌氧指示剂,在变色反应时间上表现一致。
我们应用缸内法培养粪中厌氧无芽胞菌类均获成功。一份标本中至少检出菌属1~4种,例如拟杆菌活菌数为10.70±1.43/克粪,双岐杆菌为10.69±1.17/克粪。我们在工作中发现粪中厌氧菌在“Bd”培基上菌落外表相似时,鉴定时菌种可能不同。 |
| English Abstract: |
A simple technic of cultivating anaerobes vas presented. By utilizing a large-sized desicator with suitable amount of steel wool or iron filings at the bottom of jar, & through certain chemical reactions, free O2 being absorbed, together with appropriate amount of CO2 introduced after the cultures(plates, tubes) were put into the desicator. anaerobic bacteria have been succesfully cultivated from stool specimens. There is no need of special equipment and anyone can make use of this method in an ordinary laboratory. The ‘anaerobic’ Indicator we prepared has been found comparable to Gaspak (BBL) Indicator as far as the time of discoloration was concerned.
The preliminary trials vre made with the above mentioned technic in cultivating fecal anaerobic bacterial flora indicated that at least 2-4 bact. spp. of nonsporiug strict anaerobes could be cultivated out of each specimen, the average figures for living Bacteroides per Gram of feces being 10.70±1.43; & that of Bifidobacteria 10.69±1.17. It was revedled that the growth of colonies on ‘Bd’ medium, one might find different species of anaerobic bacilli with apparently similar outward appcarance. |
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