| 杨莲芬,程尧章,姜顺求.布氏菌内毒素的类脂A抗血清制备[J].Chinese journal of Epidemiology,1983,4(6):342-344 |
| 布氏菌内毒素的类脂A抗血清制备 |
| Preliminary Study on Preparation of Immune Sera against lipid A from Brucella Endotoxin |
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| Abstract: |
| 应用精制后的16M菌内毒素酚相制品经乙酸水解制备了类脂A。经类脂A与布氏菌酸水解产物、不完全佐剂以及类脂A与不完全佐剂皮下注射和类脂A直接静脉注射三种方法免疫家兔制备了抗类脂A血清。其中静脉注射法较好。以酶联免疫吸附试验、试管凝集试验和半胱氨酸凝集试验检查了免疫血清的效价。抗类脂A血清经用布氏菌细胞吸收后,用ELISA试验检查抗体效价不变,而SAT和CYT所测效价明显下降。表明用类脂A免疫家兔不仅产生了抗类脂A的抗体,也产生了抗布氏菌细胞的抗体。 |
| English Abstract: |
| An acetic acid hydrolytic preparation of brucella lipid A was obtained from Br. melitensis 16M endotoxin. The lipid A extract could be made use of in preparing antisera by way of intravenous immunization or subcutaneous injection corporated with incomplete Freund's adjuvant in rabbits. When such antisera were absorbed by brucella cells, we found no drop in titre by means of ELISA test against lipid A antigen. This means the lipid A preparation contains very little cell surface antigen. |
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