| 王福菊,丁正荣,陈琨琳,杨进业,邓洁清,邵惠训.蚊虫人工感染乙型肝炎病毒的实验观察[J].Chinese journal of Epidemiology,1984,5(1):19-20 |
| 蚊虫人工感染乙型肝炎病毒的实验观察 |
| Experimental Observation on Infection of Mosquitoes with Hepatitis B Virus |
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| Abstract: |
| 本文介绍1982年用实验室饲养的致乏库蚊进行人工感染HBsAg阳性血。逐日采蚊用RPHA、ELISA、SPRIA三种方法检测，发现蚊虫携带HBsAg与蚊胃血消化有关。蚊虫吸血后1~4天内可检出HBsAg，血液消化后HBsAg也消失。HBsAg阳性蚊的排泄物、卵、幼虫、蛹及第一代雌、雄蚊均未检出HBsAg。说明吸血蚊的传播乙型肝炎病毒的作用，可能是通过机械性传播，而蚊虫不是媒介宿主。 |
| English Abstract: |
| Mosquitoes of the species Culex fatigans were fed artificially on blood containing HBsAg. ELIRA, SPRIA and RPHA were used for detecting HBsAg daily. It was found that HBsAg was detected for up to 1-4 days after feeding and disappeared in parallel with blood meal digestion. HBsAg was not detected in the excrete of adults, eggs,larvae, pupae and the first generation of female and male mosquitoes.There was no evidence of replication of HBsAg. It was suggested that the transmissive role of HBV by mosquitoes may be a mode of mechanic. |
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