| 纪绍忠,严玉辰,李爱芳,陶小霞,崔小英,孔令雄,赵荣辉,董必军,丘福禧.酶标葡萄球菌A蛋白组化法用于肾综合征出血热诊断的研究[J].Chinese journal of Epidemiology,1984,5(5):297-301 |
| 酶标葡萄球菌A蛋白组化法用于肾综合征出血热诊断的研究 |
| Studies on the Diagnosis of Hemorrhagic Fever with Renal Syndrome by means of An Indirect Immunoenzymatic Histochemical Method with Horseradish Peroxidase-labelled Staphlococcus Protein A |
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| Abstract: |
| 本文报告应用HRP-SPA组化法检测用4株不同来源的HFRS病毒抗原免疫的家兔血清和10例HFRS病人双份血清的抗体滴度，结果与IFA法基本一致，证明了HRP-SPA组化法的特异性。用HRP-SPA组化法和IFA法检测了不同疫区30例病人的恢复期血清，来源于黑线姬鼠和褐家鼠的Vero-E6细胞HFRS病毒抗原和7只HFRS抗原阳性黑线姬鼠肺洗液中的抗体，两法结果相同，证明了HRP-SPA组化法与IFA法一样，具有较高的灵敏性。此外，HRP-抗人IgG组化法用于HFRS的诊断也是可行的。 |
| English Abstract: |
This article reports the establishment of an indirect immunoenzymatic histochemical method with horseradish peroxidase-labelled staphylococcus protein A for the diagnosis of hemorrhagic fever with renal syndrome. Using this method, hemorrhagic fever virus antigens in Vero-E6 cells infected two strains of HFRS virus were stained. Positive and negative results were easily read under light microscope. Paired sera from 10 HFRS patients were examined and a fourfold or more increase in antibody titer was shown in all the convalescent sera. The titer of the antibody to HFRS virus were assayed in convalescent sera of 30 patients with HFRS and in rabbit immune sera against HFRS virus by indirect immunoenzymatic histochemical method with horseradish peroxidase-labelled staphylococcus protein A and by indirect immunofluorescence for comparison. The titers of antibody detected by indirect immunoenzymatic histochemical method were similar to those by indirect immunofluorescence in all sera of patients with HFRS. Sera from 30 normal individuals and 35 non-HFRS patients were all negative by both methods. These data indicate that indirect immunoenzymatic histochemical method with horseradish peroxidase-labelled staphylococcus protein A is rather sensitive and specific, and thus may be applied as a new method for the diagnosis of HFRS.
We have also used horseradish peroxidase-labelled anti-human G in substitution for horseradish peroxidase-labelled staphylococcus protein A in the assay of the titers of the antibody to HFRS virus in paired sera of 10 patients and convalescent phasesera of another 10 patients with HFRS, and compared with those by indirect immunofluorescence. The results were coincidental, indicating that an indirect immunoenzymatic histochemical method with horseradish peroxidase-labelled anti-human IgG may be used for the diagnosis of HFRS. |
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