| 汪义和,刘如星,邵芯仪,张新萍,陆群英,潘荫华.甲型病毒性肝炎的早期诊断:ELISA法检测特异性IgM抗体的研究[J].Chinese journal of Epidemiology,1985,6(2):88-91 |
| 甲型病毒性肝炎的早期诊断:ELISA法检测特异性IgM抗体的研究 |
| Early Diagnosis of Viral Hepatitis A-Study on Application of ELISA in Detection of Specific IgM Antibody |
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| Abstract: |
| 本文采用一种ELISA法检测IgM抗-HAV。当在本实验系统中加入2-ME处理的阳性血清或阳性血清结合后再用羊抗人IgM进行阻断试验,结果均可使其OD值下降约78%;甲肝流行点急性期血清IgM抗-HAV检出率高达92.3%(48/52),而恢复期血清仅达17.1%(7/41);11份急性乙肝和33份脐血清检测结果均为阴性;本法与Abbott HAVAB-M试盒检测结果符合率达86%;若以Abbott RIA法为标准,本法的敏感性和特异性分别为91.2%和80%,且重复性良好,操作简便,成太低和无放射性危害等优点。 |
| English Abstract: |
| An enzyme-linked immunosorbent assay (ELISA) for the detection of IgM anti-HAY was described. For determining the specificity of ELISA, the positive sera were treated by 2-ME or blocked by goat antiserum to human IgM, and the optical density readings of all sera declined about 78%. 52 sera from acute patients in epidemic areas of hepatitis A were tested. 48 of them (92.3%) showed positive, whereas only 7 of 41 (17.1%) sera from convalescent Patients showed positive.11 sera from Patients of acute hepatitis B and 33 sera from umbilical cords all gave negative results.The coincidence rate of the results of ELISA in this paper with that of RIA provided by Abbottkit was 86%. The sensitivity and specificity of ELISA were 91.2%and 80%, respectively. Comparing with RIA, this technique was more simple, less expensive and reproducible, and had no radiation haggard, |
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