| 淄博市卫生防疫站,张店区卫生防疫站,利津县卫生防疫站,阳信县卫生防疫站,惠民地区卫生防疫站,淄博市传染病医院.用标记SpA染色试剂与尿液协同凝集反应快速诊断伤寒病的研究[J].Chinese journal of Epidemiology,1985,6(3):170-172 |
| 用标记SpA染色试剂与尿液协同凝集反应快速诊断伤寒病的研究 |
| Use of Coagglutination Technic with Labelled SpA Stained Reagent and Urine in Rapid Diagnosis of Typhoid Fever |
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| Abstract: |
| 本文报告1981~1982年,对经血液、粪便细菌培养和肥达氏反应阳性诊断的192例伤寒病人,采用SpA菌膏吸收代替离心沉淀除去尿液中非特异性凝集物质法,与诊断血清标记SpA加美蓝染料的试剂做玻片法协同凝集反应,从中获得阳性185例,符合率为96.35%和清晰易辨的满意结果,并做了154例对照试验,建立了一个取材方便、操作简易、结果快速、敏感特异、适合流行病学调查和临床诊断伤寒病的新方法,并具有明显的经济效益。 |
| English Abstract: |
Urine samples were taken from 192 patients with typhoid fever diagnosed by blood and faeces cultures and Widal reaction. Urine from which non-specific agglutinin had been absorbed by SpA pellet was mixed on a slide with diagnostic antiserum labelled with SpA and dyed with methylene blue. Coagglutination was observed.
Positive reaction was found in 185 samples with a coincidence rate of 96.35%. Thus a simple and sensitive procedure for epidemiological survey and clinical diagnosis was established. |
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