| 于恩庶,肖巧斌.布鲁氏菌的一种简单敏感培养基[J].Chinese journal of Epidemiology,1985,6(5):307-309 |
| 布鲁氏菌的一种简单敏感培养基 |
| A Simple and Sensitive Medium for Brucella |
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| Abstract: |
利用牛肉汤所剩下的肉渣,再用胰酶消化为肉渣消化液,按氨基氮50~70mg%的浓度稀释,配成液体和固体培养基,适于各布鲁氏菌的生长,表现在出菌快,培养最初几天的菌落大,对初代分离培养,可代替肝琼脂培养基,但长期传代不适合,容易变为粗糙型。
这种培养基所需材料少,不用蛋白胨和其他营养物质,成本低,符合节约原则,具有在广大疫区推广使用的条件。 |
| English Abstract: |
After veal infusion broth being made, the residue of veal can digested by adding trypsin. Then the digestive juice can be used to make liquid and solid media.The method is the same as making other infusion broth media, but they must contain aminonitrogen from 50-70%.These media can support better growth of each biotype of Brucella. Using the media to separate the contaminants of Brucella shows the positive result sooner than using liver infusion agar.Also the colonies is larger,So, the veal residue digest broth can be used to culture Brucella primarily from infective materials instead of using liver infusion broth.
Since it only uses less materials and it needs not to add glycerine and dextrose, and it's cheap in making the medium,the media is worth being widly used in epidemic areas. |
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