芦天林,陈惟吾,贾芙容,苏年英,刘军.用微量组织培养技术测定脊髓灰质炎中和抗体[J].Chinese journal of Epidemiology,1985,6(6):356-358 |
用微量组织培养技术测定脊髓灰质炎中和抗体 |
Detection of Polio-Neutralizing Antibody by Micro-tissue Culture Technique |
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Abstract: |
1982年用微量组织培养技术对山西翼城地区0~15岁的150名儿童进行灰质炎中和抗体测定。结果I、Ⅱ、Ⅲ型中和抗体均阳性者为22.67%;各年龄组I型抗体阳性率为58.82~89.47%, Ⅱ型为0~72.50%;Ⅲ型为0~65.38%,各型抗体的滴度分布大多数在1:5~1:20之间。同时用微量组织培养技术,设备简便、节省材料和时间、基层易推广应用。 |
English Abstract: |
In 1982,150 blood samples were collected from new-born to 15-year old children at Yi-cheng prefecture, Shanxi province, and the neutralizing antibodies against poliomyelitis virus type I, Ⅱ andⅢ were detected by a micro-tissue culture technique. It was demonstrated that 22.67% of the sample had the antibodies to all 3 types of polio-virus. The antibody positive conversion rates in different age group were 58.82%~89.47%, 0~72.50% and 0~65.38% for poliovirus type I, Ⅱ and Ⅲ,respectively.The serum antibody titer of all 3 types of poliomyelitis in great majority of these children were 1:5~1:20. The micro-tissue culture technique proved a simple and cheaper method used in detection of polio-neutralizing antibodies. |
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