| 雷祚荣,曲丽云,王鲁明.酶联免疫吸附试验和反向间接血球凝集快诊金葡菌肠毒素的效果观察[J].Chinese journal of Epidemiology,1986,7(1):51-53 |
| 酶联免疫吸附试验和反向间接血球凝集快诊金葡菌肠毒素的效果观察 |
| An Observation on the Efficacy of Enzyme-Linked-Immunosorbent-Assay (ELISA) and Reversed Passive Hamagglutination (RPHA) in Rapid Detection of Staphylococcal Enterotoxin |
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| Abstract: |
| 本文用ELISA、RPHA快速诊断金黄色葡萄球菌C1、C2型肠毒素,并对某些条件进行了比较。发现5μg/ml抗体包被,1%羊血清封闭,酶标抗体1:3000~1:4000倍稀释,致敏抗体用量0.15mg/ml较合适。实验的灵敏度1.25~5.00ng/ml,特异性好,阻断实验阳性率50%以上,无论是实验污染C1、C2型肠毒素,还是金葡菌爆发的中毒食品检测都可达到毫微克水平,食品成分对检出没有干扰。检验的各种含毒食品标本,不需用酶、酸、碱、氯仿等前处理,没有假阳性。 |
| English Abstract: |
| Enzyme Linked Immunosorbent Assay (ELISA) and Reversed Passive Hamagglutination (RPHA) were used for rapid detection of staphylococcal enterotoxins Cl, C2. Some experimental conditions were compared, it was found that 5μg/ml of coating antibody,1% covering sheep serum and 1:3000~1:4000 dilution of antibody enzyme conjugate and sensitized with 0.15mg/ml of purified anti-enterotoxin immunoglobulin are more advisable. Assay sensitivity ranges from 1.25ng to 5.0ng of toxin per ml of sample.The procedure has a good specifity. The rate of blocking (inhibition) test reached more than 50%. Minute amounts of enterotoxins were added to a variety of representative foods that were usually implicated in staphylococcal food poisoning outbreakes. The toxins were consistently detectable by ELISA and RPHA at enterotoxins levels of about 2.5-12.5ng/g of food. In detection of toxin from contaminated foods, the pretreatment with enzyme, acid or base or chloroform is not necessary. No false positive reactions were encountered. |
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