| 邓化芳,马尔瞻,刘晓晖,李军,星炎.应用染色菌液微量法测百日咳抗体[J].Chinese journal of Epidemiology,1986,7(4):240-242 |
| 应用染色菌液微量法测百日咳抗体 |
| The Microtiter Agglutination Test for Detection of Pertussis Antibody by Using Stained Bacterial Suspeusion |
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| Abstract: |
| 本文介绍用染色菌液微量法与常规试管法比较检测百日咳抗体,得到相同的滴度,显示出微量法有如下优点:①仅需用少量待检血清(0.025或0.01毫升)便可检测百日咳抗体,解决了白百破制剂免疫监测中血清量不足的问题;②百日咳菌液经染色后结果较试管法容易判断;③此法重复性好;④简便易行,节约人力物力与时间。 |
| English Abstract: |
| This paper described a microtechnique for determination of pertussis agglutinin. Comparison of microtechnique with tube agglutination method, identical titres were obtained. This microtechnique showed many advantages, it required only a small amount of test serum (0.025 or 0.01ml). Bacterial suspension was stained with methylene blue, which ensured that read the titres easily. This method was reproducible well and it could be processed, large numbers of samples with minimum requirements on glassware and material, time and labour were saved. These Suggested that the improved microtechnique could be used as a tool for epidemiological, immunological surveillance of whooping cough. |
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