| 郭杏英,黄海鹰,余佩华,余哲,毛江森.甲型肝炎病毒在恒河猴中连续传代的实验观察[J].Chinese journal of Epidemiology,1987,8(5):301-304 |
| 甲型肝炎病毒在恒河猴中连续传代的实验观察 |
| An Experimental Observation on Rhesus Monkeys Continuously Transimitted with Human Hepatitis A Virus (HAV) |
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| DOI： |
| KeyWord: HAV 恒河猴 |
| English Key Word: HAV Rhesus Monkey |
| FundProject: |
| Author Name | Affiliation | | Guo Xingying | Depertment of Virology, Institute of Medical Microbiology, Zhejiang Academy of Medicine, Hangzhou | | 黄海鹰 | Depertment of Virology, Institute of Medical Microbiology, Zhejiang Academy of Medicine, Hangzhou | | 余佩华 | Depertment of Virology, Institute of Medical Microbiology, Zhejiang Academy of Medicine, Hangzhou | | 余哲 | Depertment of Virology, Institute of Medical Microbiology, Zhejiang Academy of Medicine, Hangzhou | | 毛江森 | Depertment of Virology, Institute of Medical Microbiology, Zhejiang Academy of Medicine, Hangzhou |
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| Abstract: |
| 本文报告了用人HAV直接感染恒河猴并连续传代的结果。三代共四只恒河猴感染HAV后SGPT反应曲线相似,有两个异常高峰。四只猴感染前及恢复期的双份血清测定抗-HAV,结果恢复期血清抗-HAV均阳转。抗-HAV阳转的时间,三代猴都在接种HAV后7~10周之间。第一代和第二代猴的粪便经放射免疫和IEM检查证明HAAg阳性。传代感染的成功,证明了此第1、2代猴粪便排泄HAV的事实。另外,三只人工饲养2~8个月的恒河猴抗-HAV阳性,说明还能获得自然感染。 |
| English Abstract: |
An animal model of hepatitis A was Performed with rhesus monkeys (Macaca mulatta). Three Passages of human hepatitis A virus with four infantile seronegative monkeys were proved by seroconversion and HAV fecal shedding. The primary material of HAV for transmitting to monkeys was fecal suspension extracted from a hepatitis A patient in latent phase.
All of the animals, two peaks of SGPT appeared within twenty weeks post inoculation the first peak was in about 1st-7th weeks, and the secord was arranged in 12th-16th weeks. Sera conversion of anti-HAV antibody was observed in all of four monkeys within ten weeks after inoculation. The hepatitis A virus antigen shedding in feces of first and second passage rhesus monkeys had detected by RIA and immune electron microscopy. It presented in stools of three animals was proved.
In addition, three rhesus monkeys which were manfed 2-8 months appeared positive anti-HAV reaction, It means that the rhesus monkeys could be naturally infected.
We consider that the rhesus monkeys (Macaca mulatta) may act as an animal model of hepatitis A. |
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