柏禽刺螨叮刺传播和经期、经卵传递流行性出血热病毒的实验研究

诸葛洪祥; 孟阳春; 蓝明扬; 周洪福

Abstract

诸葛洪祥,孟阳春,蓝明扬,周洪福.柏禽刺螨叮刺传播和经期、经卵传递流行性出血热病毒的实验研究[J].Chinese journal of Epidemiology,1987,8(6):336-339

柏禽刺螨叮刺传播和经期、经卵传递流行性出血热病毒的实验研究

Experimental Study on Biting, Trans-Stadial and Transovarian Transmission of Epidemic Haemorrhagic Fever Virus by Gamasid Mites Ornithonyssus bacoti

DOI：

KeyWord: 流行性出血热病毒革螨（柏禽刺螨）叮刺传播经期传递经卵传递

English Key Word: EHFV gamasid mites (Ornithonyssus bacoti) Biting transmission Trans-stadial transmission Transovarian transmission

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Author Name	Affiliation
Zhuge Hong xiang	Department of Parasitology Suzhou Medical College
et al	Department of Parasitology Suzhou Medical College
蓝明扬	Department of Parasitology Suzhou Medical College
周洪福	Department of Parasitology Suzhou Medical College

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Abstract:

首先接种EHFV苏-163株于乳小鼠,再以柏禽刺螨若螨和成螨叮刺此感染性鼠,经10天,15天和25天将这些螨及其子代若螨分别叮刺健康乳鼠,然后以间接免疫荧光技术检测,结果除10天子代组阴性外,其余均获EHF特异性荧光颗粒,而对照乳鼠阴性。与呼肠孤病毒Ⅰ、Ⅱ型抗血清呈阴性,且EHF免疫血清可阻断特异性荧光反应。因此,我们首次证明柏禽刺螨不仅能经叮刺传播,而且能经期传递和经卵传递EHF。病毒在螨体内能生存和繁殖26天以上。
由于柏禽刺螨是家鼠和大、小白鼠寄生革螨的优势种,分布广、数量大、专性吸血、经常侵袭人群,其季节消长与发病季节相符。所以我们认为,此螨在城市型和实验动物型EHF的传播和维持中起重要作用。

English Abstract:

The identified EHFV strain Su-163 was firstly inoculated into suckling mice. Then let nymphs and adults of gamasid mites (Ornithonyssus bacoti) bite the infected mice. On the 10th, 15th and 25th days, let these mites and their 2nd generation protonymph bite healthy suckling mice. The EHF antigen was tested with indireot immunofluoreseent technique. It was shown that the specific fluorescence granules were detected in all of them except the group of 2nd generation protonymph on the 10th day, while in the control & suckling mice, the reovirus types I, II were all negative, and that the specific fluorescence reaction could be blocked by EHFV immuno-serum.
Thus, we, for the first time, provided evidence that O. bacoti could transmit EHFV not only by biting, but also by trans-stadial and transovarian. The virus could survive in the mites for at least 25 days. As O. bacoti is the predominant species on rats and mice, widely in distribution, large in number, and exclusively hemophilic, and its seasonal fluctuation is in conforming with the incidence of human EHF, we consider-that it may possibly be the vector and reservoir of both urban and laboratory animal types of EHF.

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