| 毛华,李之桂,万超群.BA-ELISA和CoA法检测尿中军团菌抗原[J].Chinese journal of Epidemiology,1988,9(2):103-106 |
| BA-ELISA和CoA法检测尿中军团菌抗原 |
| Detection of Legionella Antigens in Urine by Biotin-Avidin Enzyme-Linked Immunosorbent Assay and Coagglutination Test |
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| DOI： |
| KeyWord: BA-ELISA法 CoA法 军团菌 |
| English Key Word: BA-ELISA CoA Legionnaires' Disease Bacterium |
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| Abstract: |
| 本文报告了用自己建立的BA-ELISA法和CoA法检测嗜肺性军团菌血清Ⅰ型(LP1)感染豚鼠和病人尿标本的结果。BA-ELISA法的灵敏度为0.1ng蛋白/ml,CoA法为60ng蛋白/ml。两法综合判断,军团病人的检出比为7/7。BA-ELISA法特异性较强,CoA法有4%的假阳性。 |
| English Abstract: |
This article reported a Biotin-Avidin Enzyme-Linked Immunosorbent Assay (BA-ELISA) and a Coagglutination test (CoA) to detect Legionella-Soluble antigens in urine specimens of 38 artificially infected guinea pigs and 63 pati ents. The BA-ELISA was found to be able to detect as little as 0.1 ng protein/ml, and the CoA was 60 ng protein/ml.
The urine specimens of 7 patients with Legionnaires' Disease diagnosed by indirect fluorescent assay (IFA) were found to have positive result in both tests. No false positive result was found in BA-ELISA in patients suffering from Pneumonia other than Legionellosis as well as ‘healthy’ persons. 2 patients and 2 ‘healthy’ individuals, however, revealed false positive in CoA test. |
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