| 薛小平,田红,李秋瑾,李彤.肾综合征出血热血清IgG的固相发光免疫测定[J].Chinese journal of Epidemiology,1988,9(4):238-240 |
| 肾综合征出血热血清IgG的固相发光免疫测定 |
| Detection of IgG Antibody in Serum of Hemorrhage Fever with Renal Syndrome (HFRS) by Solid-Phase Chemiluminescence Immunoassay |
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| DOI： |
| KeyWord: 固相化学发光免疫测定法 肾综合征出血热 IgG抗体 |
| English Key Word: Solid-phase Chemiluminescence Immunoassay (SCLIA) Hemorrhage Fever with Renal Syndrome (HFRS) IgG antibody |
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| Abstract: |
| 采用固相化学发光免疫测定法,检测23例出血热病人血清标本中的IgG抗体,结果均为阳性。本方法的敏感性高于间接免疫荧光法:抗体滴度较高(﹥1:3200),阳性率稍高。对15份对照血清标本重复检测1:200稀释时均为阴性。本方法重复性良好,组内CV=2.3~6.0%,n=6,组间CV=7.0%,n=3。 |
| English Abstract: |
| The solid-phase chemiluminescence immunoassay for the detection of IgG antibodies has been employed in serum of patients with HFRS. An of the 23 HFRS cases showed Positive results. Experimental results demonstrated that this method was more sensitive than immunofluorescence technique. 15 non-correspondence samples were repeatedly detected by SCLIA in control. The results were all negative, under 1:200 dilution. The repeatability of the method was better, cofficient of variation within group and among groups were 2.3-6.0% n=6 and 7.0% n=3, respectively. |
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