| 戴子森,荣新华,曹天佑.HBsAg阳性唾液传染性的研究[J].Chinese journal of Epidemiology,1988,9(6):336-338 |
| HBsAg阳性唾液传染性的研究 |
| A Study on Infectivity of HBSAg Positive Saliva |
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| DOI： |
| KeyWord: 乙型肝炎 唾液HBV DNA检测 RIA及斑点分子杂交试验 |
| English Key Word: Hepatitis B Salivary HBV-DNA RIA Spot hybridization technique |
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| Abstract: |
| 本文以RIA及斑点分子杂交试验,对80例HBsAg携带者及43例乙型肝炎病人的唾液和血清中HBsAg及HBV DNA进行了检测。结果发现,HBsAg携带者唾液中HBsAg的阳性率为38.8%,HBV DNA的阳性率仅为2.5%;乙型肝炎病人唾液中HBsAg的阳性率为51.2%,HBV DNA的阳性率为7.0%。HBsAg携带者及乙型肝炎病人血清中HBV DNA的阳性率分别为28.8%和46.5%。唾液及血清标本的斑点分子杂交试验放射自显影结果显示,唾液中HBV DNA的浓度远较血清为低。上述结果可见,HBsAg阳性唾液中HBV DNA的阳性率及其浓度均较低。据此可初步认为,HBsAg阳性唾液作为乙型肝炎传播因子的流行病学意义较小。 |
| English Abstract: |
HBsAg and HBV DNA in saliva from 80 asymptomatic HBsAg carriers and 43 HBsAg positive Hepatitis B patients wers detected by RIA and by a simple spot hybridization technique The results showed the positivity rates of HBsAg and HBV-DNA in saliva collected from HBsAg carries were 38.8% (31/80) and 2.5% (2/80) respectively, and were 51.2% (22/43) and 7.0% (3/43) respectively from Hepatitis B patients. The results of a autoradiograph from dot-blot of saliva and serum samples by simple spot hybridization showed that consistency of saliva samples wasmuch lower than that of the serum samples.
The above results, both the positivity rate and consistency of HBV DNA were low, did not support the general view that HBsAg Posive saliva was a very important vehicle in transmission of HBV.
As to the source of HBV DNA in saliva, the possibility of localization and replication of HBV in salivary gland should be further investigated. |
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