| 王继斌,赵克义,吴俊英,曹金福,李云圃.改良间接免疫荧光方法在军团病诊断上的应用[J].Chinese journal of Epidemiology,1989,10(1):44-46 |
| 改良间接免疫荧光方法在军团病诊断上的应用 |
| Modified IFA Assay in Diagnosis of Legionellosis |
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| DOI： |
| KeyWord: 军团病 改良间接免疫荧光方法 间接免疫荧光方法 灵敏性 特异性 |
| English Key Word: Legionellosis IFA Modi-fied IFA Specificity Sensitivity |
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| Abstract: |
| 本文用IFA与改良IFA法平行对Lp-I、Ⅲ~Ⅵ型与各型抗血清和有关病原体抗血清及31株肺炎病原菌与Lp各型抗血清进行交叉试验,两法结果相同。检测46例肺炎病人双份血清和116份正常人血清抗体,两法结果基本一致。证明改良IFA与原法一样,具有较高的灵敏性和特异性,可用于军团病的实验室诊断。 |
| English Abstract: |
| IFA and modified IFA assay were used for the antigen-antibody reaction between L. pneumophila type I, Ⅲ to Ⅵ and their anti-sera as well as ant卜sera of other related bacteria, and between 31 strains of pneumonic pathogens and various types of Lp-antisera. The same results were obtained by using these two methods. 46 pneumonia cases with paired sera and 116 normal sera were examined for Lp-antibody.The results obtained by both were almost the same. It was shown that the modified IFA assay was sensitivity and specific as the original one,and it could be used in laboratory for the diagnosis of Legionellosis. |
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