牛布氏菌病特异抗体的鉴别

卢德威; 王大勇; 严延生; 姚虹; 于恩庶; 尚德秋; 崔春槐; 李兰玉

Abstract

卢德威,王大勇,严延生,姚虹,于恩庶,尚德秋,崔春槐,李兰玉.牛布氏菌病特异抗体的鉴别[J].Chinese journal of Epidemiology,1989,10(3):143-145

牛布氏菌病特异抗体的鉴别

An Identification of Special Antibodes for Brucellosis

DOI：

KeyWord: 布氏菌病免疫斑点试验小肠结肠炎耶氏菌

English Key Word: Brucella Y. enterocolitica Dot-ELISA

FundProject:

Author Name	Affiliation
Lu De-Wei	Sanitary and Anti-Epidemic Station of General Bureau-State Farm of Hei Leng Jiang province
王大勇	Sanitary and Anti-Epidemic Station of General Bureau-State Farm of Hei Leng Jiang province
严延生	福建省人兽共患病研究室
姚虹	福建省人兽共患病研究室
于恩庶	福建省人兽共患病研究室
尚德秋	中国预防医学科学院流行病学微生物学研究所
崔春槐	中国预防医学科学院流行病学微生物学研究所
李兰玉	中国预防医学科学院流行病学微生物学研究所

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Abstract:

应用硝酸纤维素膜为固相的免疫斑点试验,以牛种布氏菌和0:3型小肠结肠炎耶氏菌为抗原,检查88份布病疫区牛血清,并与常规ELISA和试管凝集试验进行比较,结果表明免疫斑点试验与常规ELISA非常一致,符合率达到98%,均比试管凝集试验敏感。免疫斑点试验还能鉴别布氏菌抗体和0:9型小肠结肠炎耶氏菌抗体,是其它方法不能比的,加上方法简便、快速、稳定、容易掌握,具有在基层推广的条件,可以帮助临床诊断和流行病学调查。

English Abstract:

Appling antigens of B. abortus and Y. enterocolitica 0:3, detection 88 sera of cattle in foci of Brucellosis was carried out by Dot-ELISA on nitrocellulose filter paper, and compared with conventional ELISA and SAT. The results showed that coincident rate of Dot-ELISA and ELISA was 98%. Their sensitivity was more than SAT. In contrast with others, the Dot-ELISA sould differentiate Brucella antibody from antibody to Y. enter, 0:9. In addition, the method is a simple, Speed, stable, and very easy. The procedure sould help clinical diagnosis and epidemiological investigation.

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