| 张升,何桂铭.我国不同地理株白纹伊蚊对登革病毒的易感性[J].Chinese journal of Epidemiology,1989,10(6):348-351 |
| 我国不同地理株白纹伊蚊对登革病毒的易感性 |
| Studies on the Susceptibility among Geographic Strains of Aedes albopictus in China to Infection with Dengue Viruses |
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| DOI： |
| KeyWord: 白纹伊蚊 易感性 登革病毒 地理株 |
| English Key Word: Aedes albopictus Susceptibility Dengue virus Geographic strain |
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| Abstract: |
| 本文用棉球病毒悬液法感染白纹伊蚊,比较了我国9个不同地理株白纹伊蚊对经口感染登革病毒Ⅰ、Ⅱ和Ⅳ型的易感性。实验结果表明,我国不同地理株白纹伊蚊对经口感染登革病毒的易感性有差别。海口株对三个血清型登革病毒均较易感,北京株和成都株分别对登革病毒Ⅰ型和Ⅱ型较易感,各地理株白纹伊蚊对登革病毒Ⅱ型的易感性普遍较高。本文还用蚊胸内病毒接种技术,比较了我国9个不同地理株白纹伊蚊对胸内接种感染登革病毒Ⅰ、Ⅱ和Ⅳ型的易感性,结果胸内接种各型登革病毒的易感性未见明显差异,提示经口感染易感性的差别是中肠屏障的作用。本文所采用的蚊脑涂片间接免疫荧光技术检测蚊脑内登革病毒抗原的方法,蚊脑组织在玻片上分布较均匀,可避免蚊头外骨骼对脑组织的遮盖,似比蚊头压片法易于观察结果,有利于提高检出率。 |
| English Abstract: |
The comparative susceptibility to dengue serotype 1, 2 and 4 viruses infection of 9 geographic strains of Aedes albopictus was studied by means of experimental feeding the mosquitoes on pledgets soaked with virus-erythrocyte suspension and intrathoracic inoculation with dengue viruses.Variation in susceptibility for both dengue serotypes 1 and 2 was found among the geographic strains by feeding, but no significant difference among those by intrathoracic inoculation.It was evident that a "gut barrier" was involved.Thresholds of oral infection were not the same for dengue serotypes 1, 2 and 4. In terms of infection rate, Haikou strain was more susceptible to all three serotypes of viruses; Beijing and Chengdu strains were more susceptible to serotypes 1 and 2, respectively. All the geographic strains were more susceptible to dengue serotype 2 comparing with other two serotypes.
The mosquito head squash method for demonstrating dengue virus antigen by indirect fluorecent antibody technique was modified by mosquito brain tissue smear.This would be more accurate in detecting the dengue virus antigen in the brain tissue |
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