| 张荣珍,王佩珠,武士珍,李忠亮,张淑琼,张兰香,张东.麻诊被动血球凝集试验方法的建立及初步应用的研究[J].Chinese journal of Epidemiology,1990,11(2):103-107 |
| 麻诊被动血球凝集试验方法的建立及初步应用的研究 |
| Development of Passive Hemagglutination Assay of Measles and Utilization in Serological Diagnosis |
| Received:January 20, 1989 Revised:April 30, 1989 |
| DOI： |
| KeyWord: 麻疹 被动血球凝集试验 抗原制备 |
| English Key Word: Measles Passive hemagglutio-nation Preparation of antigen |
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| Abstract: |
| 在建立麻疹被动血球凝集试验方法中,对抗原制备的条件、致敏血球的稳定性、敏感性及特异性等方面进行了研究。实验证明,单纯超声处理未浓缩抗原,产量高,在4℃条件下保存6个月效价不变;制备的致敏血球效价稳定,批间差异小、敏感性好、特异性强,冻干致敏血球能长期保存。经与血球凝集抑制试验法同时测定354人份健康儿童血清,其麻疹抗体GMT分别为26.20和13.92,两法相关系数为0.81,具有高度相关性。 |
| English Abstract: |
| The passive hemagglutination assay (PHA) was developed. Preparation of antigen and its stability, sensitivity and specificity was studied. The result showed that the yield of ultrasonic treated antigen is high and its titer has no any change in 4℃ storage at least 1 month. The sensitized sheep erythrocyte with this antigen have a high specificity and sensitivity. The lysophilized blood cells could stored for a long time in 4℃. A comparison of PHA with hemagglutination inhibition test was carried out by examination of 354 samples of sera healthy children. The GMT of titef of antibodies of measles were 26.20 and 13.92 respectively. The result showed that the two methods were highly related. |
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