柯萨奇病毒A24变异株引起的急性出血性结膜炎快速诊断的研究

沐桂藩; 吕华; 徐如良; 顾方舟; 谭柯; 庞国祥; 魏爵阳

Abstract

沐桂藩,吕华,徐如良,顾方舟,谭柯,庞国祥,魏爵阳.柯萨奇病毒A24变异株引起的急性出血性结膜炎快速诊断的研究[J].Chinese journal of Epidemiology,1990,11(3):154-155

柯萨奇病毒A24变异株引起的急性出血性结膜炎快速诊断的研究

Study on Rapid Diagnosis of Acute Hemorrhagic Conjunctivitis Caused by Coxsackievirus A24 Variant

Received:January 11, 1989

DOI：

KeyWord: 急性出血性结膜炎柯萨奇病毒A24变异株

English Key Word: Acute Hemorrhagic Conjunctivitis (AHC) Coxsackievirus A24 Variant

FundProject:

Author Name	Affiliation
Mu Guifan	Department of Microbiology and Immunology, Faculty of Basic Medicine, Peking Union Medical College
吕华	Department of Microbiology and Immunology, Faculty of Basic Medicine, Peking Union Medical College
徐如良	Department of Microbiology and Immunology, Faculty of Basic Medicine, Peking Union Medical College
顾方舟	Department of Microbiology and Immunology, Faculty of Basic Medicine, Peking Union Medical College
谭柯	北京协和医院眼科
庞国祥	北京协和医院眼科
魏爵阳	北京协和医院眼科

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Abstract:

用免疫荧光试验检査感染组织培养细胞中的病毒抗原。5天内就可得出分离定型结果。进而用免疫荧光法直接检査眼结膜细胞涂片中柯萨奇病毒A24变异株(简称CA24v)病毒抗原,阳性率为81.3%。

English Abstract:

An pandemic oT acute hemorrhagic con-junctivitis(AHC) caused by Coxsackievirus A 24 variant (CA 24v) occurred in China in 1988. Rapid diagnosis of AHC was investigated by immunofluorescence technique. The bacteria-free conjunctival swab samples were inoculated into HeLa cell monolayer. The Virus-specific antigens in infected cells were examined by indirect immunofluorescence test. Of 35 samples, 17 was identified as CA 24v. The results of isolation and typing could be obtained with in 5 days. Further, CA 4v antigen in infected conjunctival cells were examined by indirect immunofluorescence test. Of 48 smears of conjunctival cells, 39 was positive. Positive rate were 81.3%.

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