| 俞守义,陈添弥,陈义忠,王锡琪,娄丹,陈清,杨芳娣,吴益民,周善国,罗观堤,黄君健.用二种基因探针对肠毒性大肠杆菌腹泻的初步分子流行病学研究[J].Chinese journal of Epidemiology,1990,11(3):175-178 |
| 用二种基因探针对肠毒性大肠杆菌腹泻的初步分子流行病学研究 |
| A Primary Study on Molecular Epidemiology of Diarrhea Caused by Enterotoxigenic Escherichia Coli by DNA Colony Hybridization Using Three Enterotoxigenic Gene-probes |
| Received:April 02, 1989 |
| DOI： |
| KeyWord: ETEC腹泻 分子流行病学研究 基因探针 |
| English Key Word: ETEC-diarrhea Gene-probe Molecular epidemiology |
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| Abstract: |
| 应用肠毒性大肠杆菌三种肠毒素(LT、ST-P、ST-H)基因探针对近年来从广州、武汉、四川、沈阳、湖南等部分省市搜集到的921株致急性腹泻的大肠杆菌进行DNA-DNA菌落原位杂交检测。LT198株占21.5%(198/921)、ST-H 131株占14.2%(131/921)、ST-P 54株占5.9%(54/92l),ST+LT 19株占2.1%(19/921)。表明我国ETEC腹泻病原体中以LT+ ETEC为主,其次为ST-H+郊区农村ST-P+也占相当比例。为国内ETEC腹泻的分子流行病学研究提供了基本数据和较先进的诊检工具。 |
| English Abstract: |
| 921 isolates of acute-diarrchea-indicing E. Coli strains from several provinces and cities, including Guangzhou, Wuhan.Sichuan, Shenyang and Hunan, have been collected during 1984~1989 and were identified in 1989 for detecting gents coding for these enterotoxins-LT, ST-P, ST-H by DNA colony hybridization using three enterotoxigenic gene probes.Of all the isolates homologous to thesfe genes encoding LT, ST-P, ST-H and both LT and ST were 198 (21.5%), 131 (14.2%), 54 (5.9%) and 18(2.1%), respe-ctivily.It was showed that in our country pathogenic agents of acute infectious diarrheal disease with ETEC strains first might be ETEC-LT strains, secondary ETEC-ST-H strains. Whereas, in rural districts ETEC-ST-P posses higher proportion of ETEC strains.These results provided some essential data and advanced methods for researching further molecular epidemiology of the ETEC diarrheal disease. |
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