| 鲁齐发,尚德秋,王惠秋,常炳功,张伟.粗糙型布鲁氏菌单克隆抗体的制备及应用于种型鉴定的实验研究[J].Chinese journal of Epidemiology,1990,11(5):271-273 |
| 粗糙型布鲁氏菌单克隆抗体的制备及应用于种型鉴定的实验研究 |
| An Experimental Study on Preparing Monoclonal Antibodies of rough Brucella Spp. and Identification Species Brucella |
| Received:May 01, 1989 Revised:October 05, 1989 |
| DOI： |
| KeyWord: 布鲁氏菌 单克隆抗体 酶联免疫吸附试验 |
| English Key Word: Brucella Monoclonal tibodies ELISA |
| FundProject: |
| Author Name | Affiliation | | Lu | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | Qifa | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | 王惠秋 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | 常炳功 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing | | 张伟 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing |
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| Abstract: |
| 应用首次制备的犬种及绵羊附睾种布鲁氏菌单克隆抗体对不同种型布鲁氏菌株检测的实验研究表明,两种单克隆抗体对粗糙型布鲁氏菌具有特异性,可应用于粗糙型与光滑型布鲁氏菌的鉴别,并且较之常规凝集试验,不仅可定性或定量检测,而且应用ELISA试验检查具有很髙的敏感性。 |
| English Abstract: |
| A report of preparcation of monoclonal antibodies of B. canis and B. ovis and the use of identification for rough species of Brucella was described. The results showed that the above two monoclonal antibodies were specific for rough Brucella Spp. The use of the above described monoclonal antibodies gave us the possibility to differenate rough and smooth Brucella species. The results also showed that the ELISA using monoclonal antibodies of rough Brucella Spp. was more specific than the agglutination test using routine antibodies of rough Brucella. |
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