| 邵一鸣,曾毅,陈筝,赵全壁,赵尚德,马瑛,贾曼红,杨文乔,段一娟,段松.从云南艾滋病病毒(HIV)感染者分离HIV[J].Chinese journal of Epidemiology,1991,12(3):129-135 |
| 从云南艾滋病病毒(HIV)感染者分离HIV |
| Isolation of Human Immunodeficiency Virus(HIV) in Epidemic Area of HIV infection in Yunnan Province |
| Received:October 01, 1990 Revised:December 01, 1990 |
| DOI： |
| KeyWord: 人免疫缺损病毒 病毒分离 |
| English Key Word: Human immunedeficiency virus Virus isolation |
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| Abstract: |
| 从云南人免疫缺损病毒(HIV)感染流行区感染者采血,用外周血单个核细胞(PMCs)共培养方法分离HIV,以逆转录酶测定法(RT)为检测终点。总共从24名无症状HIV抗体阳性者和一名持续性全身性淋巴腺病病人分离到10株HIV。经HIV1p 24 Elisa和HIV1 Pol和Gag基因引物多聚酶链反应(PCR)证实并鉴定所分离株均为HIV1型。与文献报道从同类人群所分离的毒株生物学特性相似,这些毒株仅在PMCs缓慢生长,不致细胞病变。目前正在改变培养条件以提高病毒滴度并对扩增的基因进行分析以寻找我国云南HIV流行株与世界其他地区HIV流行株的差异。 |
| English Abstract: |
| Blood were collected from HIV infected persons in epidemic area of HIV infection in Yunnan province for isolation of HIV.The coculture method was used for cultivating the virus and reverse transcriptase assay (RT) was the main method for detection of HIV. Of 25 seropositive, 24 asymptomatic and one PGL, 10 showed positive RT activity (>5 000 cpm/ml and with a steadily increase, some to more than 40 000 cpm/ml). The results were confirmed by the detection of HIV1 p24 Ag (ELISA)and HIV1 POL and GAG gene sequence (PCR)). In accordance with the reports from other labs, the viruses isolated from these group of persons infect only PMCs, grew slowly with gradual increase of RT activity and caused no CPE. Efforts are making, at present, to rise the virus titer with better culture system. The amplified gene sequence of the isolates are under investigating. |
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