清除莱姆病螺旋体培养物中杂菌的方法研究

万康林; 张金声; 张哲夫

Abstract

万康林,张金声,张哲夫.清除莱姆病螺旋体培养物中杂菌的方法研究[J].Chinese journal of Epidemiology,1991,12(5):299-301

清除莱姆病螺旋体培养物中杂菌的方法研究

Study on the Elimination of Bacteria from the Cultures of Lyme Disease Spirochetes (Borrelia burgdorferi)

Received:June 23, 1990 Revised:November 27, 1990

DOI：

KeyWord: 莱姆病螺旋体污染纯化

English Key Word: Lyme disease spirochetes Contamination Purification

FundProject:

Author Name	Affiliation
Wan Kanglin	Institute of Epidemiology and Microbiology, Chinese Academy of Prevetive Medicine
张金声	Institute of Epidemiology and Microbiology, Chinese Academy of Prevetive Medicine
张哲夫	Institute of Epidemiology and Microbiology, Chinese Academy of Prevetive Medicine

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Abstract:

应用抗血清法、药物法、稀释法、微孔滤膜自动过滤法和动物接种法对从蜱分离的伴有较多杂菌的H₁₁和H₂₀莱姆病螺旋体培养物和从病人分离的伴有少量杂菌的R₉莱姆病螺旋体培养物进行了纯化。抗血清法对污染严重的培养物可除去大部分杂菌,达到初步纯化。初步纯化的莱姆病螺旋体培养物和伴有少量杂菌的莱姆病螺旋体培养物,用滤膜过滤法或药物法或稀释法或动物接种法均可达到纯化目的。但过滤法最简便、效果最好。

English Abstract:

Contaminated cultures of strains H₁₁, H₂₀ from ticks, and strain R₉ from patient were purified by antisera for bacteria, chemicals, filtzation dilation and animal inoculation. Most of bacteria, were eiminated after adding antisera into contaminated strains H₁₁, H₂₀ The preliminarily decontaminated cultures of strains H₁₁, H₂₀ or those with a few bacteria such as strain R₉ could be completely purified by filtration, chemicals, dilution, or animal inoculation, but filtration is the simplest and the most effective purification method.

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