海捕毛蚶中分离到甲型肝炎病毒

李爱萍; 谢慰杖; 赵世立; 陈伟; 宋伟涛; 王宇露; 王少军

Abstract

李爱萍,谢慰杖,赵世立,陈伟,宋伟涛,王宇露,王少军.海捕毛蚶中分离到甲型肝炎病毒[J].Chinese journal of Epidemiology,1992,13(3):141-142

海捕毛蚶中分离到甲型肝炎病毒

Isolation of HAV from Sea Clam

Received:August 15, 1991 Revised:December 01, 1991

DOI：

KeyWord: HAV 毛蚶

English Key Word: HAV Sea Clam

FundProject:

Author Name	Affiliation
Li Aiping	Shandong Provincial Hygiene and Epidemic Prevention Station
谢慰杖	Shandong Provincial Hygiene and Epidemic Prevention Station
赵世立	Shandong Provincial Hygiene and Epidemic Prevention Station
陈伟	Shandong Provincial Hygiene and Epidemic Prevention Station
宋伟涛	Shandong Provincial Hygiene and Epidemic Prevention Station
王宇露	Shandong Provincial Hygiene and Epidemic Prevention Station
王少军	Shandong Provincial Hygiene and Epidemic Prevention Station

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Abstract:

应用电镜和超薄切片技术、免疫荧光(IF)试验、酶联免疫试验、核酸分子杂交技术、病毒感染滴度测定及中和试验等方法,直接从海捕毛蚶中检测和分离到三株甲肝病毒(HAV),并在人二倍体(2BS)细胞上稳定传代,感染滴度在4.0~5.0TCID₅₀/ml之间。初步结果证实,海捕毛蚶受污染并携带甲肝病毒,可成为甲肝流行的潜在危险因素。

English Abstract:

Three strains of HAV were isolated directly from clams (Area Subcrensta Lisckke) collected from sea with cell culture and identified by electron microscopy, immunofluorescent test, ELISA, HAV-cDNA hybridization and neutralization test.The strains were stably passed in 2BS cells with the titer of 4.0~5.0 TCID50/ml. Results indicated that the clams were contaminated by HAV and may cause an outbreak of hepatitis A.

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