| 肖乐义,王珊珊,徐德忠,李远贵,周华,陈友绩.固相免疫吸附血凝抑制试验快速检测甲型肝炎IgM抗体[J].Chinese journal of Epidemiology,1992,13(4):229-232 |
| 固相免疫吸附血凝抑制试验快速检测甲型肝炎IgM抗体 |
| Rapid Detection of Anti-HAV IgM by Solid-phase Immunosorbent Hemagglutination Inhibition Test |
| Received:December 13, 1991 Revised:February 01, 1992 |
| DOI： |
| KeyWord: 甲型肝炎病毒 免疫吸附技术 血凝抑制试验 |
| English Key Word: Hepatitis A virus Immunosorbent technics Hemagglutination inhibition test |
| FundProject: |
| Author Name | Affiliation | | Xian Leyi | Department of Epidemiology, Fourth Military Medical University, Xian | | 王珊珊 | Department of Epidemiology, Fourth Military Medical University, Xian | | 徐德忠 | Department of Epidemiology, Fourth Military Medical University, Xian | | 李远贵 | Department of Epidemiology, Fourth Military Medical University, Xian | | 周华 | Department of Epidemiology, Fourth Military Medical University, Xian | | 陈友绩 | Department of Epidemiology, Fourth Military Medical University, Xian |
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| Abstract: |
| 本文报告一种新建立的快速检测抗-HAV IgM的固相免疫吸附血凝抑制试验。本试验与ELISA对比检测血清标本336份,总符合率为99.4%。被检的甲肝感染者血清抗-HAV IgM滴度范围为1:20~1:327 680。阻断试验、2-ME破坏试验及非甲肝病人血清检测结果表明本试验具有良好的特异性。本试验不需标记抗体,不受类风湿因子的干扰,不需要特殊仪器,且经济简便,能在3小时内得出结果,可用作甲肝的早期诊断和流行病学监测,尤其适于基层单位应用。 |
| English Abstract: |
A solid-phase immunosorbent hemagglutination inhibition test (SPISHAI) was developed for hepatitis A virus-specific immunolobulin M (IgM) antibody
Three hundred thirty and six sera were comparatively detected with both SPISHAI and ELISA. Among them 97 sera were positive and 237 were negative with both method. The crude agreement rate was 99.4%.With SPISHAI the titers of anti-HAV IgM ranged from 1:20 to 1:327 680 among tested sera from infected individuals by HAV. The specificity of SPISHAI was confirmed by 2-ME trentment method and bloking test.The patients with non-A hepatitis all got negative results.
The SPISHAI does not require conjugated antibody and sophisticated equipment, and is not interfered with rheumatoid factor in sera. Furthermore, the result of the test can be got within 3 hours. Therefore, the SPISHAI is a cheap and simple, and could be applied for early diagnosis and epidemiological surveillance of hepatitis A in the community and in primary health care. |
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