一种用于人畜布鲁氏菌病诊断的羧化胶乳凝集试验

鲁齐发; 王晓英; 郭宝岚; 张伟

Abstract

鲁齐发,王晓英,郭宝岚,张伟.一种用于人畜布鲁氏菌病诊断的羧化胶乳凝集试验[J].Chinese journal of Epidemiology,1992,13(5):291-293

一种用于人畜布鲁氏菌病诊断的羧化胶乳凝集试验

A Carboxylated Latex Agglutination Test for the Serological Diagnosis of Humen and Animal Brucellosis

Received:July 01, 1991 Revised:September 10, 1991

DOI：

KeyWord: 布鲁氏菌病羧化胶乳凝集试验抗原抗体

English Key Word: Brucellosis Carboxylated latex agglutination test Antigen Antibody

FundProject:

Author Name	Affiliation
Lu Qifa	Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing
王晓英	Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing
郭宝岚	Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing
张伟	Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing

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Abstract:

本文介绍一种用于人畜布鲁氏菌病诊断的羧化聚苯乙烯胶乳凝集试验。所用抗原是以高浓度氯化钠液提取的一种蛋白性抗原。而且不是用传统的物理吸附法而是用化学交联法制备的,具有良好的稳定性。将化学交联胶乳和物理吸附胶乳用于部分人畜布鲁氏菌感染血清的检测结果表明,前者无论在特异性及敏感性上均优于后者。并对本试验的一些影响因素进行了讨论。

English Abstract:

A carboxylated latex agglutination test was devlopped for detecting antibodies in sera of humen and animals with Brucellosis. The latex was chemico-linked with B. melitensis 16M extracted with 5% NaCl. Comparing with physico-sorbent latex antigens in detecting antibodies in sera of humen and animals with Brucellosis, the chemicolinked latex is more stable, more specific and more senitive. In addition, preparation of chemico-linked latex and some fators affecting the result of the test were also described in the paper.

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