| 方捍华,韩秀娟,潘雯,戴斌.捕获-EIA法用于麻疹疫苗免疫效果监测的初步探讨[J].Chinese journal of Epidemiology,1992,13(5):297-299 |
| 捕获-EIA法用于麻疹疫苗免疫效果监测的初步探讨 |
| Study on the Immunization Effect of Measles Vaccine by Capture-EIA |
| Received:January 22, 1991 Revised:July 26, 1991 |
| DOI： |
| KeyWord: 麻疹疫苗 捕获-EIA 特异性IgM抗体 |
| English Key Word: Measles vaccine Capture-EIA Specific IgM antibody |
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| Abstract: |
| 用捕获-EIA法和血凝抑制试验同时检测72名麻疹疫苗初免者的特异性IgM抗体和血凝抑制抗体。结果表明,72份免疫前及7份未免疫成功者血清,两种方法检测结果完全相符(均为阴性)。65名血凝抑制抗体阳转者,捕获-EIA法检测结果的符合率为83~100%。特异性IgM抗体水平和动态与接种的疫苗病毒量相关。由此,我们认为捕获-EIA法完全可用于麻疹疫苗初免后的效果监测。 |
| English Abstract: |
| Capture-EIA was applied to detect specific IgM antibody (SIgM) to measles virus in paired sera from 72 children before and after parimary vaccinatoin and HI antibody was also tested simultaneously. The SIgM and HI antibodies were not detected in 72 prevaccinated and 7 post-inoculated sera by both the capture-EIA and HI test. Among the 65 sera-conversion samples by HI test in the 15 days after immunization, comparing with the results obtained by the capture-EIA, the comformability were 83~100%. The results showed that there was a certain relationship between the level of SIgM produced and the vaccine dose inoculated. So, the results indicated that for investigation of the effect of immunization with measles vaccine. Capture-EIA is as good as HI test. |
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