| 舒泉,毕诚,薛平,秦生巨.嗜肺性军团杆菌培养基(BALM)的研究[J].Chinese journal of Epidemiology,1992,13(5):300-303 |
| 嗜肺性军团杆菌培养基(BALM)的研究 |
| Study on the Medium (BALM) for Isolation of Legionella Pneumophila |
| Received:October 30, 1991 Revised:March 20, 1992 |
| DOI： |
| KeyWord: 嗜肺性军团杆菌 蓝藻培养基 |
| English Key Word: Legionella pneumophila BALM |
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| Abstract: |
| 蓝藻培养液能促进嗜肺性军团杆菌的生长,将此培养滤液适量加入参照硝酸铁培养基配方用国产试剂自制的基础培养基(LM)中,制成蓝藻培养基(BALM)。嗜肺性军团杆菌第6血清型标准株在BALM上形成菌落的能力优于BCYE;第1血清型标准株在两种培养基上的菌落形成能力较接近。从临床标本中分离NANJ-1株的结果表明,该菌株在BALM上的生长时间、菌落大小优于BCYE和其他几种培养基。 |
| English Abstract: |
| L.pneumophila was apparently using blue algae (cyanobacteria) extracellular products as carbon and energy sources for its proliferation. Based on this observation, a medium (BALM)in which all chemicals and reagents were made in China for isolation of L.pneumophia was developed. The recoveries of L.pneumophia serogroups 1 and 6 standard strains from contaminated air-conditional water and infected guinea pig spleens were evaluated by using two culture media:BALM and BCYE (buffered charcoal yeast extract agar).Recoveries of standard strains of L.pneumophila serogroup 1 were similar on both media, while those of L.pneumophila serogroup 6 were more efficient on BALM than on BCYE. In the process of isolation of L.pneumophila NANJ-1 strain, which was obtained from the material of tracheal lavage of a pneumonic patient in one of hospitals in Nanjing City, the recovery of this strain was also more efficient on BALM than on BCYE and other common used media. The results suggested that the use of BALM medium in place of BCYE may improve the recover of L.pneumophia from clinical and environmental specimens. |
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