| 张惠君,陈燕,黄松如,邵兰,黄敏君,宋丽玉,何似.应用抗恙虫立克次体的单抗检测恙虫立克次体抗原[J].Chinese journal of Epidemiology,1993,14(1):49-51 |
| 应用抗恙虫立克次体的单抗检测恙虫立克次体抗原 |
| Detection Antigen of Rickettsia tsutsugamushi by Using Monoclonal Antibody |
| Received:July 31, 1992 Revised:October 14, 1992 |
| DOI： |
| KeyWord: 恙虫立克次体 恙螨 单克隆抗体 斑点ELISA |
| English Key Word: Rickettsia tsutsugamushi Chigger Monoclonal antibody Dot-ELISA |
| FundProject: |
| Author Name | Affiliation | | Zhang Huijun | Beijing Tropical Medicine Research Institute, Beijing, 100050 | | 陈燕 | Beijing Tropical Medicine Research Institute, Beijing, 100050 | | 黄松如 | Beijing Tropical Medicine Research Institute, Beijing, 100050 | | 邵兰 | Beijing Tropical Medicine Research Institute, Beijing, 100050 | | 黄敏君 | Beijing Tropical Medicine Research Institute, Beijing, 100050 | | 宋丽玉 | 福建省卫生防疫站 | | 何似 | 福建省卫生防疫站 |
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| Abstract: |
| 应用斑点ELISA技术,以辣根过氧化物酶标记的抗恙虫立克次体Karp毒株的单抗分别检测了恙虫病患者血清、恙虫立克次体保种传代的小鼠血清、恙虫病流行地区的野鼠血清以及恙螨中的恙虫立克次体抗原,结果表明本法是早期诊断恙虫病和进行该病流行病学调查的一种简便而有效的手段。 |
| English Abstract: |
| Dot-ELISA was used with HRP-labelled monoclonal antibodies Kp3 (IgG) and Kp4-6 (IgM) against antigen of the Karp strain of Rickettsia tsutsugamushi to detect the antigen of R. tsutsugamushi. The positive rate of sera of 56 acute scrub typhus patients and 37 wild rats caught from the countryside of the endemic area as well as 29b atches of chiggers collected from the wild rats were 92.9%. 100% and 89.7%, respectively. No cross reaction was found with antigens of other rickettsia groups such as R. mooseri, R. prowazeki, R. burneti and R. rickettsi. In patients suf fering from acute scrub typhus the antigen of R. tsutsugamushi could be detected earlier in the course of illness than the antibody. This method is very sensitive that an amount of antigen of 6.7ng/μl can be detected. It is easy to perform and the results can be read by naked eye. |
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