| 鲁齐发,武素怀,王晓英,郭宝岚,张伟,李兰玉,马烨,何利华.布鲁氏菌和O:9型小肠结肠炎耶氏菌两种抗体鉴别的实验研究[J].Chinese journal of Epidemiology,1993,14(5):283-286 |
| 布鲁氏菌和O:9型小肠结肠炎耶氏菌两种抗体鉴别的实验研究 |
| A Preliminary Study on Serological Differentiation between Antibody against Brucella and that against Yersinia enterocolitica Serotype O:9 |
| Received:January 30, 1993 |
| DOI： |
| KeyWord: 布鲁氏菌 小肠结肠炎耶氏菌 酶连接免疫吸附测定 抗原 抗体 |
| English Key Word: Brucella Yersinia enterocolitica ELISA Antigen Antibody |
| FundProject:本项研究为国家自然科学基金资助项目 |
| Author Name | Affiliation | | Lu Qifa | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 武素怀 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 王晓英 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 郭宝岚 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 张伟 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 李兰玉 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 马烨 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 何利华 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 |
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| Abstract: |
| 采用SAT、ELISA及DIBA分别对6种布鲁氏菌及耶氏菌O:9型感染的兔血清抗体的检查结果表明,无论用布鲁氏菌104M、耶氏菌O:9型菌凝集抗原做SAT检查或用布鲁氏菌16M超声波破碎抗原做ELISA检查均对布鲁氏菌与耶氏菌O:9型的兔血清抗体无鉴别意义;而用16M可溶性蛋白抗原、外膜蛋白抗原以及用耶氏菌O:3型可溶性蛋白抗原、外膜蛋白抗原做ELISA或DIBA均能对布鲁氏菌与耶氏菌O:9型的兔血清抗体进行鉴别。在实际检测时,以同时用16M及O:3型外膜蛋白抗原做ELISA检查为宜。 |
| English Abstract: |
| Standard agglutination test (SAT), enzyme-linked immunosorbent assay (ELISA), dot immunobinding assay (DIBA) have been developed for the detection of antibodies against 6 strains of Brucella and that against Yersinia enterocolitica Serotype O:9. The results showed that differentiation could be made between Brucella and Yersinia O:9 when 16M SPA, OMP and Y O:3 SPA. OMP were used by ELISA, but not when 104M and YO:9 antigens were used by SAT. So, 16M and YO:3 OMP antigens were better for detection of antibodies against Brucella and Yersinia O:9 by ELISA. |
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