| 王云飞,刘佩莉,俞顺章,钱来娣.宫颈癌危险因素的流行病学研究[J].Chinese journal of Epidemiology,1994,15(2):103-106 |
| 宫颈癌危险因素的流行病学研究 |
| Epidemiological Study on Risk Factors of Cervical Cancer |
| Received:January 28, 1993 Revised:May 14, 1993 |
| DOI： |
| KeyWord: 癌，宫颈 人乳头瘤病毒 疱疹病毒Ⅱ型 人巨细胞病毒 斑点杂交 |
| English Key Word: Cervical cancer Human papillomavirus Herpes simplex virus type 2 Human cytomegalovirus hominis Dot blot hybridization |
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| Abstract: |
宫颈癌病因复杂,多数研究者认为是多因素综合作用的结果。本文通过病例-对照研究,应用分子及血清流行病学方法,探讨引起宫颈癌的危险因素。
应用HPV6/11、HPV16/18探针,通过斑点分子杂交技术,检测宫颈癌和对照宫颈组织DNA中的感染,结果病例组和对照组HPV6/11阳性率分别为24.6%、27.5%,差别无显著性,HPV16/18阳性率分别为45.6%、9.8%,差别有显著性;应用PCR技术检测两组宫颈组织DNA中HSV-2感染,结果病例组和对照组阳性率分别为28.1%,7.8%,Logistic回归平衡年龄因素后差别无显著性;应用ELISA方法检测血清中HCMV抗体阳性率,结果病例组和对照组IgM阳性率分别为12.3%、5.9%,差别无显著性。用多因素Logistic回归平衡各因素间的作用,最后分析结果表明,文化程度、产次、HPV16/18感染与宫颈癌的发生有关,其OR值分别为0.57、1.85、31.96。 |
| English Abstract: |
The relationship between cervical cancer and risk factors including virus infection of cervix was studied with methods of molecular and serological epidemiology. DNA extracted from cervical biopsies taken from 57 cases and 51 controls were tested for HPV6/11, HPV16/18 by Dot-blot hybridization and for HSV-2 by PCR.Sera were detected for HCMV-IgM by ELISA.The results and risk factors were analyzed by logistic regression model.
The multiple logistic regression model results showed that HPV16/18 infection was an important risk factor, the infection rate was significantly higher in the group of cervical cancer, the.OR was 31.96.Other risk factors were the educational level and the number of birth, the OR were 0.57 and 1.85, respectively. The difference of HSV-2 infection of cervix and HCMV-IgM in serum was not significant between the two groups. |
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