| 余传信,万超群,邓长英.嗜肺军团菌聚合酶链反应检测方法及其应用研究[J].Chinese journal of Epidemiology,1994,15(2):117-120 |
| 嗜肺军团菌聚合酶链反应检测方法及其应用研究 |
| Studies on the Detection of Legionella pneumophila by the Application of Polymerase Chain Reaction (PCR) |
| Received:May 03, 1993 |
| DOI： |
| KeyWord: 军团菌 聚合酶链反应 |
| English Key Word: Legionella PCR |
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| Abstract: |
| 根据嗜肺军团菌基因组DNA的种特异性DNA片段序列,合成一对引物进行聚合酶链反应(PCR).经琼脂糖凝胶电泳、EB染色结果表明,一条87cbp的核苷酸区带为嗜肺军团菌1~14血清型菌株所共有。PCR检测水中军团菌,其敏感性为350cfu/ml,而用同位素标记探针、斑点杂交法检测,其敏感性为43cfu/ml.PCR检测人工感染嗜肺军团茵的豚鼠组织标本,阳性率为83.3%,而细菌分离培养的阳性率仅为26.6%.在现场调查中,用PCR法初步验证了一起由Lp10引起的军团菌病爆发。上述结果表明,PCR法能快速、敏感、特异性检测嗜肺军团菌感染. |
| English Abstract: |
| According to the sequence of genomic DNA fragment of Legionella pneumophila, the PCR was performed with a pair of artificial synthesized primer.Results of agarose electrophoresis and EB staining showed that there was a 870 bp band shared by serogroups 1~14 of L.pneumophila. The sensitivity of PCR in detecting Legionella from water was 350cfu/ml, however, the specific DNA probe labeled with 32p was 43cfu/ml by blot hybridization. The positive rate of tissue specimens from infected guinea-pigs with Legionella pneumophila was 83.3% by PCR detection, and only 26.6% by bacteriological culture method. An outbreak caused by Lp10 was verified by PCR. The result showed that the PCR could detect the infection of Legionella rapidly, specifically and sensitively. |
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