我国伤寒沙门氏菌的分子流行病学特征I.我国部分地区伤寒沙门氏菌的多位点酶电泳研究

徐文斌; 祁国明; 刘延清

Abstract

徐文斌,祁国明,刘延清.我国伤寒沙门氏菌的分子流行病学特征I.我国部分地区伤寒沙门氏菌的多位点酶电泳研究[J].Chinese journal of Epidemiology,1994,15(4):218-222

我国伤寒沙门氏菌的分子流行病学特征I.我国部分地区伤寒沙门氏菌的多位点酶电泳研究

Molecular Epidemiological Characteristics of Salmonella typhi Strains Isolated in China I. Analysis of Salmonella typhi Strains Isolated in China by Multilocus Enzyme Electrophoresis

Received:July 12, 1993 Revised:August 07, 1993

DOI：

KeyWord: 伤寒沙门氏菌多位点酶电泳分子流行病学

English Key Word: Salmonella typhi Multilocus enzyme electrophoresis (MEE) Molecular epidemiology

FundProject:本题目为国家自然科学基金资助课题

Author Name	Affiliation
Xu Wenbin	Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206
Qi Guoming	Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206
Liu Yanqing	Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206

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Abstract:

通过聚丙烯酰胺凝胶电泳(PAGE)分析了我国部分伤寒高发省份在不同年代分离到的335株伤寒菌的七种染色体编码的多位点代谢酶。结果发现这七个酶位点均有多态性。其平均等位基因数为7.4(2~19);平均遗传多态度为0.498(0.198~0.833)。335株伤寒菌的七个酶位点电泳结果通过排序和数值分类可分为147个不同的电泳型(ETs)。根据对ETs进行聚类分析以及聚类时不相似系数曲线变化情况,把147个ETs归属于22个不同的亚克隆系,12个克隆系。进一步的分析发现,不同地区、不同年代、不同来源的菌株有其规律性,1988年的江苏、浙江菌株大都来自同一克隆系;1990年的广西菌株和1990年的部分湖北菌株也为同一个克隆系菌株。不同年代分离的湖南和贵州菌株之间具有较近的亲缘性;而新疆菌株则有相对的独立性。同时结果还显示同一次伤寒爆发或流行中分离到的菌株均来自一个亚克隆系。

English Abstract:

Multilocus enzyme analysis by polyacrylamide gel electrophoresis (PAGE) was used to analyse 335 isolates of S.typhi from several provinces where high morbidity of typhoid fever was observed in recent years. We analysed 7 metabolic enzymes encoded by chromosome. The results showed that all the enzyme loci were polymorphic. The average alleles per locus was 7.4 (2-19):the mean genetic diversity per locus was 0.498 (0.198 0.883). There were 147 distinctive electrophoretic types (ETs) in 335 isolates. With cluster analysis by the average-linkage method, we found that there were 22 subclones, 12 clones among all isolates studied. Most isolates in 1988 from both Jiangsu and Zhejiang provinces belonged to one clone; the isolates from Guangxi Province in 1990 and parts of Hubei isolates belonged to one clone; the isolates from Hunan and Guizhou provinces during several-year period were homogeneous. However, the isolates from Xinjiang Autonomous Region were remarkebly different from other provinces, beloning to a particular clone. We also observed that the isolates from an outbreak or epidemic were from a one subclone.

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