| 徐海峰,杨为松,崔龙洙,朱德生,白雪帆.用ELISA检测大鼠血清中肾综合征出血热病毒特异性抗体[J].Chinese journal of Epidemiology,1994,15(4):227-230 |
| 用ELISA检测大鼠血清中肾综合征出血热病毒特异性抗体 |
| The Study on ELISA Methods for Detection of Specific Antibodies against Hemorrhagic Fever with Renal Syndrome Virus in Rat Sera |
| Received:December 22, 1993 Revised:March 07, 1994 |
| DOI： |
| KeyWord: 肾综合征出血热 抗体 酶联免疫吸附试验 |
| English Key Word: Hemorrhagic fever with renal syndrome Antibody Enzyme-linked immunosorbent assay |
| FundProject: |
| Author Name | Affiliation | | Xu Haifeng | Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xian 710038 | | Yang Weisong | Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xian 710038 | | Cui Longzhu | Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xian 710038 | | 朱德生 | 第四军医大学动物实验中心 | | 白雪帆 | Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xian 710038 |
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| Abstract: |
| 本研究应用单克隆抗体(McAb)技术,建立了三种检测大鼠血清中肾综合征出血热病毒(HFRSV)特异性抗体的ELISA。通过与间接免疫荧光法(IFAT)对比试验发现,三种ELISA在检出大鼠血清HFRSV特异性抗体的阳性率和敏感性上均明显高于IFAT试验,并且ELISA与IFAT总检出符合率为88.5%。此外还发现由于引入小鼠抗大鼠Kappa轻链McAb,抗体捕捉ELISA和间接夹心ELISA具有早期诊断的特性。这些方法的建立对野生及实验动物的HFRSV流行病学监测及检疫有一定价值。 |
| English Abstract: |
| Three ELISA methods were developed to detect specific antibodies against hemorrhagic fever with renal syndrome virus (HFRSV) in rats sera. It was shown that all three kinds of McAb-ELISA were specific, sensitive and simple for the detection of HFRSV antibodies in rat sera. |
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