| 段佩若,张励力,李晓光,刘华,李凤秀,张会川,林京.应用系列呼吸道病毒单克隆抗体快速检测分泌物中的病毒抗原[J].Chinese journal of Epidemiology,1994,15(5):308-310 |
| 应用系列呼吸道病毒单克隆抗体快速检测分泌物中的病毒抗原 |
| Rapid Detection for Antigens of Respiratory Tract Viruses by Using Serizes of Monoclonal Antibody |
| Received:July 16, 1993 |
| DOI： |
| KeyWord: 呼吸道病毒感染 单克隆抗体 |
| English Key Word: Infections of respiratory tract viruses Monoclonal antibody |
| FundProject: |
| Author Name | Affiliation | | Duan Peiruo | Department of Clinical Experiment, 262 Hospital, Beijing, 100088 | | Zhang Lili | Department of Clinical Experiment, 262 Hospital, Beijing, 100088 | | Li Xiaoguang | Department of Clinical Experiment, 262 Hospital, Beijing, 100088 | | 刘华 | Department of Clinical Experiment, 262 Hospital, Beijing, 100088 | | 李凤秀 | Department of Clinical Experiment, 262 Hospital, Beijing, 100088 | | 张会川 | Department of Clinical Experiment, 262 Hospital, Beijing, 100088 | | 林京 | Department of Clinical Experiment, 262 Hospital, Beijing, 100088 |
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| Abstract: |
| 应用系列主要呼吸道病毒﹝呼吸道合胞病毒(RSV),甲型流感(FluA)、乙型流感(FluB)、副流感1、3型(PIV1.3)、副流感2型(PIV2)病毒共6种﹞单克隆抗体(McAb),联接APAAP桥联技术,深入部队对260例呼吸道感染青年战士的鼻咽部脱落细胞中的抗原进行检测。检出FluA抗原18例,PIV2抗原10例,FluB和RSV各1例。以上结果经间接免疫荧光(IFA)以及阻断和替代试验,证实系列单克隆抗体检出抗原结果是特异的。笔者认为本方法是临床快速诊断以及流行病学调查的有效手段。 |
| English Abstract: |
| A series of monoclonal Antibodies (McAb) against six main respiratory tract viruses (RTV)-respiratory syncytial virus (RSV), Influenza A virus (FluA), influenza B virus (FluB), parainfluenza virus type 1.2.3 (PIV 1.2.3) were developed, and were used in APAAP bridge-enzyme assay to rapidly detect the antigens of RTV. Total 260 samples of nasopharyngeal exfoliated cells were collected from young men in Beijing Army suffered from respiratory tract virus infections. The results showed that 18 cases were tested with FluA antigen; 10 with PIV2 antigen and 1 with FluB and another with RSV antigen, respectively. All the positive samples were determined by indirect immunofluorescence assay (IFA) and blocking test and replacement test. It is suggested that the method used in the study be effective in clinical rapid diagnosis and epidemiological survey. |
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