| 张健之,范明远,毕德增,孙福祥,程昭祥,兰福春,崔文富,孙秀英,只相国,林碧瑚.多聚酶链反应技术在斑点热群立克次体流行病学调查中的应用[J].Chinese journal of Epidemiology,1995,16(1):25-28 |
| 多聚酶链反应技术在斑点热群立克次体流行病学调查中的应用 |
| The Application of PCR to Epidemiological Study on Spotted Fever Group Rickettsiae |
| Received:June 01, 1994 Revised:September 10, 1994 |
| DOI： |
| KeyWord: 多聚酶链反应（PCR） 斑点热群立克次体 蜱 啮齿动物 |
| English Key Word: Polymerase Chain reaction(PCR) Spotted fever group rickettsiae(SFGR) Tick Rodent |
| FundProject:本研究为国家自然科学基金、卫生部科研基金资助课题 |
| Author Name | Affiliation | | Zhang. Jian-zhi | Department of Rickettsiology, Institute of Epidemiology & Microbiology, Chinese Academy of Preventive Medicine, Beijing, 102206 | | Fan Ming-yuan | Department of Rickettsiology, Institute of Epidemiology & Microbiology, Chinese Academy of Preventive Medicine, Beijing, 102206 | | Bi De-zeng | Department of Rickettsiology, Institute of Epidemiology & Microbiology, Chinese Academy of Preventive Medicine, Beijing, 102206 | | 孙福祥 | 黑龙江省虎林县卫生防疫站 | | 程昭祥 | 黑龙江省虎林县卫生防疫站 | | 兰福春 | 饶河县卫生防疫站 | | 崔文富 | 绥芬河卫生检疫局 | | 孙秀英 | 绥芬河市卫生防疫站 | | 只相国 | 黑龙江省卫生防疫站 | | 林碧瑚 | 海南医学院 |
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| Abstract: |
| 作者首次用来自立氏立克次体(R. rickettsii, Rr)190KDa蛋白抗原基因序列设计的一对引物扩增从黑龙江、河北、海南、北京采集的蜱、蜱卵、幼蜱、蜱粪及啮齿动物脏器中斑点热群立克次体DNA。从上述标本中均检测出了斑点热群立克次特异的532bp大小的DNA片段,该结果部分与同期进行的病原分离结果一致。故认为,PCR技术是一种快速、敏感、特异的斑点热群立克次体流行病学调查方法。 |
| English Abstract: |
| It was the first time that a primer pairs derived from the 190KDa protein antigen gene of R. rickettsii were used to amplify SFGR DNA in ticks, tick ova,larva,tick faeces and rodent organs which were collected in Hebei,Heilongjiang,Hainan nd Beijing. A 532bp fragment was respectively amplified from above samples.The results were partially in concordance with data obtained through rickettsiae isolation. It was suggested that PCR is a rapid,specific,sensitive and practical method for detection of SFGR in endemic foci. |
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