我国伤寒沙门氏菌的分子流行病学特征:Ⅱ.部分伤寒沙门氏菌的16S rRNA基因多态性

徐文斌; 祁国明; 刘延清

Abstract

徐文斌,祁国明,刘延清.我国伤寒沙门氏菌的分子流行病学特征:Ⅱ.部分伤寒沙门氏菌的16S rRNA基因多态性[J].Chinese journal of Epidemiology,1995,16(1):29-35

我国伤寒沙门氏菌的分子流行病学特征:Ⅱ.部分伤寒沙门氏菌的16S rRNA基因多态性

Study on the Characteristics of Molecular Epidemiology for Salmonella typhi Isolated in China Ⅱ. Analysis of 16S rRNA Gene Restriction Patterns (16S rRNA GRP) of S. typhi Isolated in China

Received:August 18, 1994 Revised:September 20, 1994

DOI：

KeyWord: 伤寒沙门氏菌 16S rRNA GRP 分子流行病学

English Key Word: S. typhi 16S rRNA GRP Molecular epidemiology

FundProject:本研究为国家自然科学基金资助课题

Author Name	Affiliation
Xu Wen-bin	Instltute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206
Qi Guo-ming	中国预防医学科学院
Liu Yan-qing	Instltute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206

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Abstract:

利用PCR扩增标记的Dig-dUTP-16S rRNA基因为探针,分析我国不同时间和地区分离的119株伤寒沙门氏菌和1株鼠伤寒沙门氏菌染色体经PstI消化后的16S rRNA基因限制性图谱。结果发现,各菌株的杂交片段范围为7.0~26.5kb,每个菌株有5~10条杂交带不等。通过对每个菌株的杂交结果进行数值分类,119株伤寒沙门氏菌可分为38个RTs,其中新疆伊犁1991年流行菌株和大连1990年爆发菌株大部分(13/20)为同一RT;从国内各高发省份分离的一些流行株也有相同的RT;而一些地区的散发菌株具有独特的RT;鼠伤寒沙门氏菌的RT则更为特别。对39个RTs进行聚类分析发现:国内的一些流行菌株,爆发菌株在遗传距离0.55处聚成一大类;而散发菌株,非流行菌株则在0.70处聚成另一类。此外,从健康带菌者分离的菌株251所具有的RT单成一类。鼠伤寒沙门氏菌的距离更远。

English Abstract:

16S rRNA gene,a highly conservative gene in molecular evolution,was labeled with digoxigenin -11-dUTP by PCR. Using it as a probe,we investigated the 16S rRNA gene restriction patterns (16S rRNA GRP) of S. typhi after their chromosomes were digested with Pst Ⅰ. Results show that the Pst Ⅰ 16S rRNA GRP appears obvious polymorphism in strains of distinctive sources. The fragments containing 16S rRNA gene are sized 7.0-26.5kb in 16S rRNA GRP of Pst Ⅰ. 119 strains can be divided into 38 ribotypes. Most of the strains causing outbreaks from Dalian in 1990 and strains isolated during the pandemic from Yili, Xinjiang in 1991 have the same ribotype;some ribotypes contain epidemic strains which are from different geographic areas;some strains from sporadic cases have their unique ribotypes. We also find the 16S rRNA GRP with Pst Ⅰ of S. typhimurium rather different from that of S. typhi. Data from further analysis of Pst Ⅰ ribotypes by mean-linkage clustering method show that pandemic strains,outbreak strains and epidemic strains cluster at 0. 55 in genetic distance;nonepidemic strains and strains from sporadic cases gather to form another cluster at 0.70 in genetic distance. Ribotype of strain 251, which was isolated forman asymptomatic carrier is different from the two clusters. The ribotype of S. typhimurium is apparently far distant, comparing with that of S. typhi.

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