| 鲁齐发,王晓英,郝宗宇,宋桂梅,张伟,郭宝岚,李兰玉,冯立忠,严泽民,张殿柱.应用羧化胶乳凝集试验诊断人、畜布鲁氏菌病的研究[J].Chinese journal of Epidemiology,1995,16(2):92-94 |
| 应用羧化胶乳凝集试验诊断人、畜布鲁氏菌病的研究 |
| Study on Carboxylated Latex Agglutination Test for Serodiagnosis of Human and Animal Brucellosis |
| Received:June 14, 1994 Revised:July 15, 1994 |
| DOI： |
| KeyWord: 布鲁氏菌病 羧化胶乳凝集试验 抗布鲁氏菌免疫球蛋白G、M抗体 |
| English Key Word: Brucellosis Carboxylated latexagglutination test IgG and IgM antibodies to brucella |
| FundProject:本项研究为国家自然科学基金资助项目 |
| Author Name | Affiliation | | Lu Qi-fa | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | Wang Xiao-ying | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | Hao Zong-yu | 河南省地方病防治研究所 | | 宋桂梅 | 黑龙江省卫生防疫站 | | 张伟 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 郭宝岚 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 李兰玉 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 冯立忠 | 山西省卫生防疫站 | | 严泽民 | 青海省地方病防治研究所 | | 张殿柱 | 青海省兽医总站 |
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| Abstract: |
| 应用以化学交联法制备的布鲁氏菌16M抗原-羧化胶乳制剂的羧化胶乳凝集试验(LAT)、试管凝集试验(SAT)、虎红平板凝集试验(RBPT)以及酶联免疫吸附试验(ELISA)、半胱氨酸凝集试验(CYT),对在四省布鲁氏菌病疫区收集的部分人、畜血清中布氏菌抗体进行对比检测。结果表明,LAT较之RBPT具有更高的特异性;与SAT相比,无论阳性或阴性符合率均较一致。另外,LAT可检测血清中IgG、IgM两类布氏菌特异性抗体。因而本试验对人、畜布病的诊断具有特异、敏感和简便的优点,更宜在基层推广应用。 |
| English Abstract: |
A carboxylated latex agglutination test was developed for detecting antibodies in sera from 372 human and animal brucellosis. Of which 94 sera from human was detected by ELISA. The latex was chemical-linked with B. melitensis 16M and extracted with 5% NaCl. Comparing with RBPT in detecting antibodies with brucellosis, the LAT was more stable and specific; the positive or negative results detected by LAT were consistent with those by SAT and ELISA.
LAT may detect both IgG and IgM antibodies to Brucella in Human and animal. It is concluded that LAT is stable, specific, sensitive and practicible for the serodiagnosis of brucellosis. |
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