| 杨世明,杨枨林,安仁鸣,解华.消毒剂破坏乙、丙型病毒性肝炎血清标志物的初步观察[J].Chinese journal of Epidemiology,1995,16(3):154-156 |
| 消毒剂破坏乙、丙型病毒性肝炎血清标志物的初步观察 |
| Preliminary Observation on the Destructive Effect of Chemical Disinfectants in serum Markers of Hepatitis B and C |
| Received:January 16, 1994 Revised:April 06, 1994 |
| DOI： |
| KeyWord: HBV Anti-HCV ELISA |
| English Key Word: HBV Anti-HCV ELISA |
| FundProject: |
| Author Name | Affiliation | | Yang Shi-ming | Blood bank, Tang Du Hospital, Fourth Military Medical Universty, Xian, 710038 | | Yang Chang-lin | Blood bank, Tang Du Hospital, Fourth Military Medical Universty, Xian, 710038 | | An Ren-ming | Blood bank, Tang Du Hospital, Fourth Military Medical Universty, Xian, 710038 | | 解华 | Blood bank, Tang Du Hospital, Fourth Military Medical Universty, Xian, 710038 |
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| Abstract: |
| 笔者采用氯仿、40%甲醛溶液、3%双氧水及75%乙醇与HBsAg、HBeAg和抗-HCV阳性血清作用5、15、30及60分钟后,用ELISA法检测是否转为阴性,以了解消毒剂对上述阳性血清的破坏效果。结果:氯仿对HBsAg和抗-HCV阳性血清破坏效果好;40%甲醛溶液对HBsAg、HBeAg及抗-HCV阳性血清均破坏;3%双氧水对HBeAg有破坏作用,而75%乙醇对HBsAg、HBeAg及抗-HCV阳性血清作用上述时间后,检测时仍呈阳性反应。笔者只是初步观察,有关消毒剂的浓度、效果及作用机理有待迸一步观察和探讨。 |
| English Abstract: |
Chloroform, 40% formaldehyde, 3% hydrogen peroxide solution and 75% alcohol were used to act on hepatitis B surface antigen (HBeAg), hepatitis B e antigen (HBaAg) and anti-hepatitis C virus antibody (Anti-HCV) positive serum for 5, 15, 30 and over 60 minutes. They were examined by enzyme-linked immunosorbent assay and observed for the effect.
The destructive effect of chloroform on HBsAg and anti-HCV positive derum was marked. It was discovered that 40% formaldehyde solution destroyed HBsAg, HBeAg, and anti-HCV positive serm, while 75% alcohol also showed destructive effect on HBsAg, HBeAg and anti-HCV positive serum. |
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