| 林成水,曾昭鸿,曾凝梅,郭维植.国内产肠毒素大肠杆菌的发现和所进行的调查研究[J].Chinese journal of Epidemiology,1996,17(5):264-267 |
| 国内产肠毒素大肠杆菌的发现和所进行的调查研究 |
| The Detection and Study on Enterotoxigenic Escherichia coli in China |
| Received:June 05, 1996 Revised:June 25, 1996 |
| DOI： |
| KeyWord: 产肠毒素大肠杆菌 霍乱样腹泻 影响因素 |
| English Key Word: Enterotoxigenic Escherichia coli cholerae-like diarrhea Effect factor |
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| Abstract: |
| 作者于1974年在国内首先从霍乱样腹泻病人中检出产肠毒素大肠杆菌(ETEC)。霍乱样腹泻病人ETEC检出率高达36.48%,急性腹泻病人检出率为20.18%,外环境和海产品检出率为19.74%。产不耐热肠毒素(LT)菌株以37~42℃培养4天产毒量最多;兔肠结扎时,注入兔肠段的培养液或无菌肠毒素液,在肠段中作用24小时积液量最多,且每次试验都出现阳性反应。用5cm或10cm兔肠结扎段检测LT,其结果相似;每5cm肠段注入培养菌液或无菌肠毒素液,以1.00~3.00ml效果较好。pH<4.0或温度>50℃时,对LT有明显破坏作用。 |
| English Abstract: |
| In this paper, some strains of ETEC were first detected from the stools of the patients with cholerae-like diarrhea in China in 1974. With a detected rate 36.48% of ETEC Detected rates weve 20.18% from patients with acute diarrhea, 19.74% from environment and sea foods. ETEC produced most of LT toxin in culture medium when the they were incubated at 37-42℃ for 4 days. When Rabbit ileal loop test (RILT) was performed with the culture of ETEC or with germfree culture supernatant,a large quantity of intestinal fluid in response to LT was accumulated at 24 hour and showed positive reaction at every test. Similar results were qained by using 5cm or 10cm ileal loop of RILT for LT detectoing. Better effects were noticed through injection of 1.0-3.0ml culture of ETEC or germ-free enterotoxin in per 5cm ileal loop. LT could be destroyed by heating at>50℃ or pH<4.0. |
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