| 陈敏,范明远,徐国民,张健之,毕德增,王乾海,侯建林,范志杰,徐金祥.用PCR/RFLP技术对黑龙江沿岸蜱类和鼠类中北亚蜱传斑点热的检测[J].Chinese journal of Epidemiology,1997,18(1):5-7 |
| 用PCR/RFLP技术对黑龙江沿岸蜱类和鼠类中北亚蜱传斑点热的检测 |
| Detection of North-Asia Tick-Borne Spotted Fever in Ticks and Rodents along the Heilongjiang River-side by Restriction Fragment Length Polymorphism of PCR Products |
| Received:September 23, 1996 Revised:October 11, 1996 |
| DOI： |
| KeyWord: 北亚蜱传斑点热 PCR/RFLP |
| English Key Word: North Asia tick borne spotted fever PCR/RFLP |
| FundProject:本课题为卫生部科学研究基金资助项目 |
| Author Name | Affiliation | | Chen Min | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | Fan Ming-yuan | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | Xu Guo-min | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 张健之 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 毕德增 | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 | | 王乾海 | 黑龙江省卫生防疫站 | | 侯建林 | 黑河市卫生防疫站 | | 范志杰 | 黑河市卫生防疫站 | | 徐金祥 | 逊克县卫生防疫站 |
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| Abstract: |
| 为了解黑龙江沿岸部分地区斑点热自然疫源地存在情况，作者用PCR/RFLP的方法检测该地区蜱类及鼠类中的斑点热群（spotted fever group，SFG）立克次体。结果从该地区的森林革蜱、嗜群血蜱、黑线姬鼠、东方田鼠、麝鼠及棕背中均扩增出了斑点热群立克次体的特异片段，而对斑疹伤寒立克次体、恙虫病立克欢体、Q热立克次体则为阴性。PCR产物经PstⅠ及RsaⅠ酶切后发现它们的酶切图谱与西伯利亚立克次体完全相同，而有别于其它斑点热群立克次体标准株。作者认为黑龙江沿岸调查点可能存在北亚蜱传斑点热的自然疫源地。 |
| English Abstract: |
| In order to elucidate the natural foci of North-Asia tick-borne spotted fever along the bank of Heilongjiang river, we used PCR/RFLP to detect spotted fever group rickettsiae in ticks and rodents. The results showed that the wild samples of Dermacentor silvarum, Haemaphysalis concinna and Apodemus agrarius, Microtus fortis, Clethrionomys rufocanus and Ondatra zibethica were all positive with amplification, but typhus rickettsiae, tsutsugamushi fever rickettsiae and Q fever rickettsiae were all negative Futher RFLP analysis of amplified products with PstI and Rsal demonstrated that their retriction endonuclease profiles were identical to Rickettsia sibirica, but were different from the other prototype strains of SFG rickettsiae, suggesting the possible existance of natural foci of North-Asia tick borne spotted fever in these areas. |
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