| 时曼华,梁中兴,WJ Terpstra,李春好,张承峰,李文斌,聂一新,张立新,李俊华,傅桂明,RA Hartskeerl,VDK Herman.我国耕牛钩端螺旋体带菌和排菌状况调查[J].Chinese journal of Epidemiology,1997,18(1):12-14 |
| 我国耕牛钩端螺旋体带菌和排菌状况调查 |
| Investigation on the Rate of Urinary Excretion of Leptospires among Cattle Naturally Infected with Leptospira interrogans |
| Received:September 07, 1996 Revised:October 05, 1966 |
| DOI： |
| KeyWord: 聚合酶链反应 钩端螺旋体病 |
| English Key Word: Polymerase chain reaction Leptospirosis |
| FundProject:本文为欧共体和中华人民共和国卫生部科研基金资助课题 |
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| Abstract: |
| 来源不同地区的牛尿经聚合酶链反应（PCR）检测和分离培养，PCR产物凝胶电泳阳性率11%，PCR产物Southern－blot阳性率13%，分离培养阳性率3.1%。不同地区牛尿标本PCR检测结果差异明显。结果表明，我国某些地区的耕牛钩端螺旋体排菌率非常高，其中以七日热和澳洲群为主。说明耕牛是这些地区钩体病的主要传染源。PCR和培养分离结果的比较显示，PCR是构体病传染源调查的一种灵敏、特异、快速和简便的方法。 |
| English Abstract: |
| Urine samples of Leptospires from cattle were detected by polymerase chain reaction (PCR)and isolation. Positive rates by Southern-blot and agarose gel electrophoresis of PCR amplification productions were 13% and 11% respectively. Positive rate of isolation was 3.1%. Various positive rates by PCR for cattle urine from different areas were discovered. Average rate of urihary excretion of Leptospires among cattle that naturally infected with Leptospira interrogans was 13.2%. Data showed that cattle was a major source of infection of Leptospirosis in some parts of China, According to the comparsion of results between PCR and isolation, we believe that PCR is a sensitive, rapid and simple method for the investigation on source of infection of Leptospirosis. |
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